Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 594733..595532 Replicon chromosome
Accession NZ_CP080126
Organism Escherichia coli strain PK8568

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KZY03_RS02895 Protein ID WP_000347267.1
Coordinates 594733..595197 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZY03_RS02900 Protein ID WP_001307405.1
Coordinates 595197..595532 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZY03_RS02865 (589734) 589734..590168 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZY03_RS02870 (590186) 590186..591064 - 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KZY03_RS02875 (591054) 591054..591833 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZY03_RS02880 (591844) 591844..592317 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZY03_RS02885 (592340) 592340..593620 - 1281 WP_000681922.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZY03_RS02890 (593869) 593869..594678 + 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
KZY03_RS02895 (594733) 594733..595197 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZY03_RS02900 (595197) 595197..595532 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZY03_RS02905 (595681) 595681..597252 - 1572 WP_219805076.1 galactarate dehydratase -
KZY03_RS02910 (597627) 597627..598961 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZY03_RS02915 (598977) 598977..599747 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T210957 WP_000347267.1 NZ_CP080126:c595197-594733 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T210957 NZ_CP104647:c4585908-4585801 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT210957 WP_001307405.1 NZ_CP080126:c595532-595197 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210957 NZ_CP104647:4585956-4586015 [Escherichia coli]
GTCTGGTTTCAAGATTAGCCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References