Detailed information of TA system TA210946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3488155..3488849
• Replicon: chromosome
• Accession: NZ_CP080121
• Organism: Escherichia coli strain PK8217

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KZO72_RS17005
• Protein ID: WP_001263493.1
• Coordinates: 3488155..3488553 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KZO72_RS17010
• Protein ID: WP_000554757.1
• Coordinates: 3488556..3488849 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3483815)	3483815..3483895	-	81	NuclAT_8	-	-
- (3483815)	3483815..3483895	-	81	NuclAT_8	-	-
- (3483815)	3483815..3483895	-	81	NuclAT_8	-	-
- (3483815)	3483815..3483895	-	81	NuclAT_8	-	-
KZO72_RS16975 (3483155)	3483155..3484399	-	1245	WP_000189541.1	esterase FrsA	-
KZO72_RS16980 (3484491)	3484491..3484949	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KZO72_RS16985 (3485210)	3485210..3486667	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
KZO72_RS16990 (3486724)	3486724..3487245	-	522	Protein_3346	peptide chain release factor H	-
KZO72_RS16995 (3487244)	3487244..3487447	-	204	Protein_3347	RtcB family protein	-
KZO72_RS17000 (3487693)	3487693..3488145	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KZO72_RS17005 (3488155)	3488155..3488553	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KZO72_RS17010 (3488556)	3488556..3488849	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KZO72_RS17015 (3488901)	3488901..3489956	-	1056	WP_001226164.1	DNA polymerase IV	-
KZO72_RS17020 (3490027)	3490027..3490812	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KZO72_RS17025 (3490784)	3490784..3492496	+	1713	Protein_3353	flagellar biosynthesis protein FlhA	-
KZO72_RS17030 (3492720)	3492720..3493217	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T210946 WP_001263493.1 NZ_CP080121:c3488553-3488155 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T210946 NZ_CP104647:2658956-2659129 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT210946 WP_000554757.1 NZ_CP080121:c3488849-3488556 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT210946 NZ_CP104647:2658632-2658928 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6