Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 648451..649178 Replicon chromosome
Accession NZ_CP080121
Organism Escherichia coli strain PK8217

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZO72_RS03170 Protein ID WP_000550189.1
Coordinates 648451..648765 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZO72_RS03175 Protein ID WP_000560266.1
Coordinates 648762..649178 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO72_RS03150 (644609) 644609..645595 - 987 WP_219808338.1 Gfo/Idh/MocA family oxidoreductase -
KZO72_RS03155 (645674) 645674..646366 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KZO72_RS03160 (646443) 646443..646946 - 504 WP_001300832.1 M48 family metallopeptidase -
KZO72_RS03165 (647031) 647031..648167 + 1137 WP_000018683.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZO72_RS03170 (648451) 648451..648765 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZO72_RS03175 (648762) 648762..649178 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZO72_RS03180 (649223) 649223..651241 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZO72_RS03185 (651667) 651667..654018 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T210930 WP_000550189.1 NZ_CP080121:648451-648765 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T210930 NZ_CP104645:c4568080-4567973 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT210930 WP_000560266.1 NZ_CP080121:648762-649178 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT210930 NZ_CP104645:4568133-4568194 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References