210928

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 5103827..5104429 Replicon chromosome
Accession NZ_CP080120
Organism Escherichia coli strain M24

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag KZX72_RS24750 Protein ID WP_000897302.1
Coordinates 5104118..5104429 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZX72_RS24745 Protein ID WP_000356397.1
Coordinates 5103827..5104117 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX72_RS24715 (5099134) 5099134..5100063 + 930 WP_000027696.1 formate dehydrogenase accessory protein FdhE -
KZX72_RS24720 (5100245) 5100245..5100487 - 243 WP_001068514.1 CopG family transcriptional regulator -
KZX72_RS24725 (5100777) 5100777..5101625 + 849 WP_001038650.1 hypothetical protein -
KZX72_RS25850 (5101650) 5101650..5102390 + 741 WP_000608806.1 hypothetical protein -
KZX72_RS24735 (5102575) 5102575..5102793 - 219 WP_001251293.1 CopG family transcriptional regulator -
KZX72_RS24740 (5103190) 5103190..5103468 - 279 WP_001296612.1 hypothetical protein -
KZX72_RS24745 (5103827) 5103827..5104117 - 291 WP_000356397.1 NadS family protein Antitoxin
KZX72_RS24750 (5104118) 5104118..5104429 - 312 WP_000897302.1 hypothetical protein Toxin
KZX72_RS24755 (5104658) 5104658..5105566 + 909 WP_001385591.1 alpha/beta hydrolase -
KZX72_RS24760 (5105630) 5105630..5106571 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
KZX72_RS24765 (5106616) 5106616..5107053 - 438 WP_000560981.1 D-aminoacyl-tRNA deacylase -
KZX72_RS24770 (5107050) 5107050..5107922 - 873 WP_000920762.1 virulence factor BrkB family protein -
KZX72_RS24775 (5107916) 5107916..5108515 - 600 WP_001296610.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T210928 WP_000897302.1 NZ_CP080120:c5104429-5104118 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T210928 NZ_CP104645:4112218-4112373 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT210928 WP_000356397.1 NZ_CP080120:c5104117-5103827 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT210928 NZ_CP104645:c4112206-4112148 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References