210920

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3839977..3840656 Replicon chromosome
Accession NZ_CP080120
Organism Escherichia coli strain M24

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag KZX72_RS18830 Protein ID WP_000057523.1
Coordinates 3840354..3840656 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag KZX72_RS18825 Protein ID WP_000806442.1
Coordinates 3839977..3840318 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX72_RS18815 (3836221) 3836221..3837153 - 933 WP_000883041.1 glutaminase A -
KZX72_RS18820 (3837415) 3837415..3839919 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
KZX72_RS18825 (3839977) 3839977..3840318 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
KZX72_RS18830 (3840354) 3840354..3840656 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZX72_RS18835 (3840789) 3840789..3841583 + 795 WP_000365147.1 TraB/GumN family protein -
KZX72_RS18840 (3841787) 3841787..3842266 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
KZX72_RS18845 (3842290) 3842290..3843090 + 801 WP_000439798.1 hypothetical protein -
KZX72_RS18850 (3843087) 3843087..3843590 + 504 WP_000667000.1 hypothetical protein -
KZX72_RS18855 (3843628) 3843628..3845280 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T210920 WP_000057523.1 NZ_CP080120:c3840656-3840354 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T210920 NZ_CP104645:2643079-2643252 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT210920 WP_000806442.1 NZ_CP080120:c3840318-3839977 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT210920 NZ_CP104645:2642755-2643051 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References