Detailed information of TA system TA210920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3839977..3840656
• Replicon: chromosome
• Accession: NZ_CP080120
• Organism: Escherichia coli strain M24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: KZX72_RS18830
• Protein ID: WP_000057523.1
• Coordinates: 3840354..3840656 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: KZX72_RS18825
• Protein ID: WP_000806442.1
• Coordinates: 3839977..3840318 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX72_RS18815 (3836221)	3836221..3837153	-	933	WP_000883041.1	glutaminase A	-
KZX72_RS18820 (3837415)	3837415..3839919	+	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
KZX72_RS18825 (3839977)	3839977..3840318	-	342	WP_000806442.1	HigA family addiction module antitoxin	Antitoxin
KZX72_RS18830 (3840354)	3840354..3840656	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZX72_RS18835 (3840789)	3840789..3841583	+	795	WP_000365147.1	TraB/GumN family protein	-
KZX72_RS18840 (3841787)	3841787..3842266	+	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
KZX72_RS18845 (3842290)	3842290..3843090	+	801	WP_000439798.1	hypothetical protein	-
KZX72_RS18850 (3843087)	3843087..3843590	+	504	WP_000667000.1	hypothetical protein	-
KZX72_RS18855 (3843628)	3843628..3845280	-	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T210920 WP_000057523.1 NZ_CP080120:c3840656-3840354 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T210920 NZ_CP104645:2643079-2643252 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT210920 WP_000806442.1 NZ_CP080120:c3840318-3839977 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT210920 NZ_CP104645:2642755-2643051 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3