Detailed information of TA system TA210909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2088496..2088998
• Replicon: chromosome
• Accession: NZ_CP080120
• Organism: Escherichia coli strain M24

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KZX72_RS09750
• Protein ID: WP_000767819.1
• Coordinates: 2088744..2088998 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KZX72_RS09745
• Protein ID: WP_001259253.1
• Coordinates: 2088496..2088747 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX72_RS09720 (2083674)	2083674..2084741	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KZX72_RS09725 (2084741)	2084741..2085811	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KZX72_RS09730 (2085808)	2085808..2087112	-	1305	WP_001296211.1	histidinol dehydrogenase	-
KZX72_RS09735 (2087118)	2087118..2088017	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KZX72_RS09740 (2088163)	2088163..2088213	-	51	WP_001364200.1	his operon leader peptide	-
KZX72_RS09745 (2088496)	2088496..2088747	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KZX72_RS09750 (2088744)	2088744..2088998	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KZX72_RS09755 (2089081)	2089081..2089905	+	825	WP_000754737.1	SDR family oxidoreductase	-
KZX72_RS09760 (2089951)	2089951..2090880	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KZX72_RS09765 (2091095)	2091095..2091157	+	63	WP_010723108.1	membrane protein YoeI	-
KZX72_RS09770 (2091147)	2091147..2092505	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KZX72_RS09775 (2092722)	2092722..2093180	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2092722..2093180	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T210909 WP_000767819.1 NZ_CP080120:2088744-2088998 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T210909 NZ_CP104643:c4463970-4463686 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT210909 WP_001259253.1 NZ_CP080120:2088496-2088747 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT210909 NZ_CP104643:c4464202-4463960 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3