Detailed information of TA system TA210901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4576996..4577498
• Replicon: chromosome
• Accession: NZ_CP080119
• Organism: Escherichia coli strain M45

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KZX76_RS22620
• Protein ID: WP_000767819.1
• Coordinates: 4576996..4577250 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KZX76_RS22625
• Protein ID: WP_001259253.1
• Coordinates: 4577247..4577498 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX76_RS22595 (4572814)	4572814..4573272	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
KZX76_RS22600 (4573489)	4573489..4574847	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KZX76_RS22605 (4574837)	4574837..4574899	-	63	WP_010723108.1	membrane protein YoeI	-
KZX76_RS22610 (4575114)	4575114..4576043	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KZX76_RS22615 (4576089)	4576089..4576913	-	825	WP_000754737.1	SDR family oxidoreductase	-
KZX76_RS22620 (4576996)	4576996..4577250	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KZX76_RS22625 (4577247)	4577247..4577498	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KZX76_RS22630 (4577781)	4577781..4577831	+	51	WP_001364200.1	his operon leader peptide	-
KZX76_RS22635 (4577977)	4577977..4578876	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KZX76_RS22640 (4578882)	4578882..4580186	+	1305	WP_001296211.1	histidinol dehydrogenase	-
KZX76_RS22645 (4580183)	4580183..4581253	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KZX76_RS22650 (4581253)	4581253..4582320	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4572814..4573272	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T210901 WP_000767819.1 NZ_CP080119:c4577250-4576996 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T210901 NZ_CP104643:1188126-1188524 [Salmonella enterica]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT210901 WP_001259253.1 NZ_CP080119:c4577498-4577247 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT210901 NZ_CP104643:1187899-1188126 [Salmonella enterica]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3