Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 683567..684366 Replicon chromosome
Accession NZ_CP080119
Organism Escherichia coli strain M45

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KZX76_RS03240 Protein ID WP_000347251.1
Coordinates 683902..684366 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KZX76_RS03235 Protein ID WP_001296435.1
Coordinates 683567..683902 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX76_RS03220 (679352) 679352..680122 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KZX76_RS03225 (680138) 680138..681472 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KZX76_RS03230 (681847) 681847..683418 + 1572 WP_001273763.1 galactarate dehydratase -
KZX76_RS03235 (683567) 683567..683902 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZX76_RS03240 (683902) 683902..684366 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZX76_RS03245 (684421) 684421..685230 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZX76_RS03250 (685479) 685479..686759 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZX76_RS03255 (686782) 686782..687255 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZX76_RS03260 (687266) 687266..688045 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZX76_RS03265 (688035) 688035..688913 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZX76_RS03270 (688931) 688931..689365 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T210875 WP_000347251.1 NZ_CP080119:683902-684366 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T210875 NZ_CP104639:c5308481-5308378 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT210875 WP_001296435.1 NZ_CP080119:683567-683902 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210875 NZ_CP104639:5308340-5308611 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References