Detailed information of TA system TA210855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2667335..2667837
• Replicon: chromosome
• Accession: NZ_CP080118
• Organism: Escherichia coli strain M70

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KZX75_RS12440
• Protein ID: WP_000767819.1
• Coordinates: 2667583..2667837 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KZX75_RS12435
• Protein ID: WP_001259253.1
• Coordinates: 2667335..2667586 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX75_RS12410 (2662513)	2662513..2663580	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KZX75_RS12415 (2663580)	2663580..2664650	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KZX75_RS12420 (2664647)	2664647..2665951	-	1305	WP_001296211.1	histidinol dehydrogenase	-
KZX75_RS12425 (2665957)	2665957..2666856	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KZX75_RS12430 (2667002)	2667002..2667052	-	51	WP_001364200.1	his operon leader peptide	-
KZX75_RS12435 (2667335)	2667335..2667586	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KZX75_RS12440 (2667583)	2667583..2667837	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KZX75_RS12445 (2667920)	2667920..2668744	+	825	WP_000754737.1	SDR family oxidoreductase	-
KZX75_RS12450 (2668790)	2668790..2669719	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KZX75_RS12455 (2669934)	2669934..2669996	+	63	WP_010723108.1	membrane protein YoeI	-
KZX75_RS12460 (2669986)	2669986..2671344	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KZX75_RS12465 (2671561)	2671561..2672019	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2671561..2672019	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T210855 WP_000767819.1 NZ_CP080118:2667583-2667837 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T210855 NZ_CP104639:c1305576-1305262 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT210855 WP_001259253.1 NZ_CP080118:2667335-2667586 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT210855 NZ_CP104639:c1305265-1304849 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3