Detailed information of TA system TA210847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 503813..504415
• Replicon: chromosome
• Accession: NZ_CP080118
• Organism: Escherichia coli strain M70

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: KZX75_RS02335
• Protein ID: WP_000897302.1
• Coordinates: 504104..504415 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZX75_RS02330
• Protein ID: WP_000356397.1
• Coordinates: 503813..504103 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX75_RS02300 (499120)	499120..500049	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
KZX75_RS02305 (500231)	500231..500473	-	243	WP_001068514.1	CopG family transcriptional regulator	-
KZX75_RS02310 (500763)	500763..501611	+	849	WP_001038650.1	hypothetical protein	-
KZX75_RS25075 (501636)	501636..502376	+	741	WP_000608806.1	hypothetical protein	-
KZX75_RS02320 (502561)	502561..502779	-	219	WP_001251293.1	CopG family transcriptional regulator	-
KZX75_RS02325 (503176)	503176..503454	-	279	WP_001296612.1	hypothetical protein	-
KZX75_RS02330 (503813)	503813..504103	-	291	WP_000356397.1	NadS family protein	Antitoxin
KZX75_RS02335 (504104)	504104..504415	-	312	WP_000897302.1	hypothetical protein	Toxin
KZX75_RS02340 (504644)	504644..505552	+	909	WP_001385591.1	alpha/beta hydrolase	-
KZX75_RS02345 (505616)	505616..506557	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZX75_RS02350 (506602)	506602..507039	-	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
KZX75_RS02355 (507036)	507036..507908	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KZX75_RS02360 (507902)	507902..508501	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T210847 WP_000897302.1 NZ_CP080118:c504415-504104 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T210847 NZ_CP104638:c3460066-3459755 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT210847 WP_000356397.1 NZ_CP080118:c504103-503813 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT210847 NZ_CP104638:c3459754-3459464 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: S1P416