Detailed information of TA system TA210825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1911185..1911687
• Replicon: chromosome
• Accession: NZ_CP080117
• Organism: Escherichia coli strain OV24

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KZX74_RS08945
• Protein ID: WP_000767819.1
• Coordinates: 1911433..1911687 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KZX74_RS08940
• Protein ID: WP_001259253.1
• Coordinates: 1911185..1911436 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX74_RS08915 (1906363)	1906363..1907430	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KZX74_RS08920 (1907430)	1907430..1908500	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KZX74_RS08925 (1908497)	1908497..1909801	-	1305	WP_001296211.1	histidinol dehydrogenase	-
KZX74_RS08930 (1909807)	1909807..1910706	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KZX74_RS08935 (1910852)	1910852..1910902	-	51	WP_001364200.1	his operon leader peptide	-
KZX74_RS08940 (1911185)	1911185..1911436	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KZX74_RS08945 (1911433)	1911433..1911687	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KZX74_RS08950 (1911770)	1911770..1912594	+	825	WP_000754737.1	SDR family oxidoreductase	-
KZX74_RS08955 (1912640)	1912640..1913569	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KZX74_RS08960 (1913784)	1913784..1913846	+	63	WP_010723108.1	membrane protein YoeI	-
KZX74_RS08965 (1913836)	1913836..1915194	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KZX74_RS08970 (1915411)	1915411..1915869	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1915411..1915869	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T210825 WP_000767819.1 NZ_CP080117:1911433-1911687 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T210825 NZ_CP104629:c2645537-2645202 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT210825 WP_001259253.1 NZ_CP080117:1911185-1911436 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT210825 NZ_CP104629:c2645785-2645537 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGTTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3