Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 709060..709859 Replicon chromosome
Accession NZ_CP080117
Organism Escherichia coli strain OV24

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KZX74_RS03455 Protein ID WP_000347251.1
Coordinates 709060..709524 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KZX74_RS03460 Protein ID WP_001296435.1
Coordinates 709524..709859 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX74_RS03425 (704061) 704061..704495 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZX74_RS03430 (704513) 704513..705391 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZX74_RS03435 (705381) 705381..706160 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZX74_RS03440 (706171) 706171..706644 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZX74_RS03445 (706667) 706667..707947 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZX74_RS03450 (708196) 708196..709005 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZX74_RS03455 (709060) 709060..709524 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZX74_RS03460 (709524) 709524..709859 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZX74_RS03465 (710008) 710008..711579 - 1572 WP_001273763.1 galactarate dehydratase -
KZX74_RS03470 (711954) 711954..713288 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KZX74_RS03475 (713304) 713304..714074 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T210822 WP_000347251.1 NZ_CP080117:c709524-709060 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T210822 NZ_CP104629:c1638884-1638781 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT210822 WP_001296435.1 NZ_CP080117:c709859-709524 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210822 NZ_CP104629:1638743-1639014 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References