Detailed information of TA system TA210809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4289235..4289737
• Replicon: chromosome
• Accession: NZ_CP080116
• Organism: Escherichia coli strain Dog168

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KZX73_RS20630
• Protein ID: WP_000767819.1
• Coordinates: 4289483..4289737 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KZX73_RS20625
• Protein ID: WP_001259253.1
• Coordinates: 4289235..4289486 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZX73_RS20600 (4284413)	4284413..4285480	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KZX73_RS20605 (4285480)	4285480..4286550	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KZX73_RS20610 (4286547)	4286547..4287851	-	1305	WP_001296211.1	histidinol dehydrogenase	-
KZX73_RS20615 (4287857)	4287857..4288756	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KZX73_RS20620 (4288902)	4288902..4288952	-	51	WP_001364200.1	his operon leader peptide	-
KZX73_RS20625 (4289235)	4289235..4289486	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KZX73_RS20630 (4289483)	4289483..4289737	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KZX73_RS20635 (4289820)	4289820..4290644	+	825	WP_000754737.1	SDR family oxidoreductase	-
KZX73_RS20640 (4290690)	4290690..4291619	+	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KZX73_RS20645 (4291834)	4291834..4291896	+	63	WP_010723108.1	membrane protein YoeI	-
KZX73_RS20650 (4291886)	4291886..4293244	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KZX73_RS20655 (4293461)	4293461..4293919	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4293461..4293919	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T210809 WP_000767819.1 NZ_CP080116:4289483-4289737 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T210809 NZ_CP104627:787675-788085 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT210809 WP_001259253.1 NZ_CP080116:4289235-4289486 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT210809 NZ_CP104627:787428-787694 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3