Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3053809..3054536 Replicon chromosome
Accession NZ_CP080116
Organism Escherichia coli strain Dog168

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZX73_RS15000 Protein ID WP_000550189.1
Coordinates 3053809..3054123 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZX73_RS15005 Protein ID WP_000560269.1
Coordinates 3054120..3054536 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX73_RS14980 (3049966) 3049966..3050952 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
KZX73_RS14985 (3051031) 3051031..3051723 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
KZX73_RS14990 (3051800) 3051800..3052303 - 504 WP_001295542.1 M48 family metallopeptidase -
KZX73_RS14995 (3052388) 3052388..3053524 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZX73_RS15000 (3053809) 3053809..3054123 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZX73_RS15005 (3054120) 3054120..3054536 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZX73_RS15010 (3054581) 3054581..3056599 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZX73_RS15015 (3056825) 3056825..3059176 - 2352 WP_000695432.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T210806 WP_000550189.1 NZ_CP080116:3053809-3054123 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T210806 NZ_CP104623:2762-3043 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT210806 WP_000560269.1 NZ_CP080116:3054120-3054536 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT210806 NZ_CP104623:2521-2772 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References