Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3012029..3012828 Replicon chromosome
Accession NZ_CP080116
Organism Escherichia coli strain Dog168

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KZX73_RS14780 Protein ID WP_000347251.1
Coordinates 3012029..3012493 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KZX73_RS14785 Protein ID WP_001296435.1
Coordinates 3012493..3012828 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX73_RS14750 (3007030) 3007030..3007464 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZX73_RS14755 (3007482) 3007482..3008360 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZX73_RS14760 (3008350) 3008350..3009129 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZX73_RS14765 (3009140) 3009140..3009613 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZX73_RS14770 (3009636) 3009636..3010916 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZX73_RS14775 (3011165) 3011165..3011974 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZX73_RS14780 (3012029) 3012029..3012493 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZX73_RS14785 (3012493) 3012493..3012828 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZX73_RS14790 (3012977) 3012977..3014548 - 1572 WP_001273763.1 galactarate dehydratase -
KZX73_RS14795 (3014923) 3014923..3016257 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KZX73_RS14800 (3016273) 3016273..3017043 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T210805 WP_000347251.1 NZ_CP080116:c3012493-3012029 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T210805 NZ_CP104622:c57559-57278 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT210805 WP_001296435.1 NZ_CP080116:c3012828-3012493 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210805 NZ_CP104622:c57800-57549 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References