Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 33815..34542 Replicon plasmid pWW14A-IncFIB/IncHI1B
Accession NZ_CP080102
Organism Klebsiella quasipneumoniae strain WW-14A

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag KZX49_RS29075 Protein ID WP_011251285.1
Coordinates 34231..34542 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZX49_RS29070 Protein ID WP_011251286.1
Coordinates 33815..34234 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX49_RS29040 (KZX49_29020) 29283..30251 + 969 WP_219803904.1 IS5 family transposase -
KZX49_RS29045 (KZX49_29025) 30514..30642 + 129 Protein_29 integrase core domain-containing protein -
KZX49_RS29050 (KZX49_29030) 30687..31019 - 333 WP_228751160.1 hypothetical protein -
KZX49_RS29055 (KZX49_29035) 31077..32057 - 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
KZX49_RS29060 (KZX49_29040) 32103..32369 - 267 WP_228751161.1 hypothetical protein -
KZX49_RS29065 (KZX49_29045) 32700..33668 - 969 WP_074422534.1 IS5 family transposase -
KZX49_RS29070 (KZX49_29050) 33815..34234 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
KZX49_RS29075 (KZX49_29055) 34231..34542 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
KZX49_RS29080 (KZX49_29060) 34747..35184 - 438 Protein_36 DDE-type integrase/transposase/recombinase -
KZX49_RS29085 (KZX49_29065) 35298..35636 + 339 WP_023288266.1 transposase -
KZX49_RS29090 (KZX49_29070) 35633..35980 + 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
KZX49_RS29095 (KZX49_29075) 36030..37568 + 1539 WP_219804392.1 IS66 family transposase -
KZX49_RS29100 (KZX49_29080) 37698..38447 - 750 WP_219804394.1 SDR family oxidoreductase -
KZX49_RS29105 (KZX49_29085) 38544..38885 + 342 WP_032611701.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..96771 96771
- inside IScluster/Tn - - 11622..37568 25946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T210776 WP_011251285.1 NZ_CP080102:c34542-34231 [Klebsiella quasipneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T210776 NZ_CP104613:c4448923-4448816 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT210776 WP_011251286.1 NZ_CP080102:c34234-33815 [Klebsiella quasipneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT210776 NZ_CP104613:4448971-4449037 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTTATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References