Detailed information of TA system TA021077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 7097..7599
• Replicon: plasmid pYptb32953
• Accession: NC_006154
• Organism: Yersinia pseudotuberculosis IP 32953

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q663E5
• Locus tag: YPTB_RS21930
• Protein ID: WP_011191405.1
• Coordinates: 7345..7599 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q663E6
• Locus tag: YPTB_RS21925
• Protein ID: WP_011191404.1
• Coordinates: 7097..7348 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YPTB_RS21905	2572..3189	+	618	WP_011191400.1	lytic transglycosylase domain-containing protein	-
YPTB_RS21910	3194..3487	+	294	WP_011191401.1	TrbC/VirB2 family protein	-
YPTB_RS21915	3506..6253	+	2748	WP_011191402.1	VirB3 family type IV secretion system protein	-
YPTB_RS21920	6318..7031	+	714	WP_011191403.1	type IV secretion system protein	-
YPTB_RS21925	7097..7348	+	252	WP_011191404.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
YPTB_RS21930	7345..7599	+	255	WP_011191405.1	Txe/YoeB family addiction module toxin	Toxin
YPTB_RS21935	7718..7963	+	246	WP_011191406.1	EexN family lipoprotein	-
YPTB_RS21940	7975..9015	+	1041	WP_011191407.1	type IV secretion system protein	-
YPTB_RS21945	9186..9869	+	684	WP_011191408.1	type IV secretion system protein	-
YPTB_RS21950	9869..10816	+	948	WP_011191409.1	P-type conjugative transfer protein VirB9	-
YPTB_RS21955	10813..12045	+	1233	WP_011191410.1	TrbI/VirB10 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..27702	27702

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10200.73 Da
• Isoelectric Point: 8.8170

>T21077 WP_011191405.1 NC_006154:7345-7599 [Yersinia pseudotuberculosis IP 32953]
MKIVFSSQSWEDYLYWQQTDKKILKRINELIKDIQRMPFEGKGKPEPLKYNLSGFWSRRITEEHRLVYEVADDSVLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T21077 NC_006154:7345-7599 [Yersinia pseudotuberculosis IP 32953]
ATGAAAATAGTCTTTTCGAGTCAATCATGGGAAGACTATCTTTATTGGCAGCAAACCGATAAAAAAATCCTTAAGCGTAT
CAATGAGCTGATTAAAGATATTCAGCGAATGCCGTTTGAAGGAAAAGGAAAACCAGAGCCATTGAAATATAACCTCTCAG
GATTTTGGTCTCGTCGCATTACAGAAGAACATCGATTAGTTTATGAAGTTGCCGATGACAGCGTATTGATAGCGGCTTGT
CGATATCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9256.56 Da
• Isoelectric Point: 4.7996

>AT21077 WP_011191404.1 NC_006154:7097-7348 [Yersinia pseudotuberculosis IP 32953]
MRTVNYSEARQNLAATMVKTVEDRAPVLITRQNGSACVLMSLEEYESLEETAYLMRSPANAMNLMSSIEELRGGKGTERK
LAE

Nucleotide

• Length: 252 bp

>AT21077 NC_006154:7097-7348 [Yersinia pseudotuberculosis IP 32953]
ATGAGAACAGTAAATTACAGCGAAGCCCGTCAAAATCTGGCGGCAACAATGGTTAAAACAGTGGAAGATCGGGCACCCGT
ACTTATCACCCGTCAAAATGGTTCTGCGTGTGTGCTGATGTCTCTTGAAGAGTATGAATCGCTTGAAGAGACCGCATACT
TAATGCGTTCGCCAGCAAACGCAATGAACCTCATGTCGTCGATTGAAGAATTACGGGGTGGGAAAGGTACTGAAAGGAAA
CTGGCTGAATGA

Structures

Toxin

• AlphaFold DB: Q663E5

Antitoxin

• AlphaFold DB: Q663E6