Detailed information of TA system TA210744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4490805..4491559
• Replicon: chromosome
• Accession: NZ_CP080091
• Organism: Salmonella enterica strain SLR1_8245

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: KZY40_RS21720
• Protein ID: WP_000558168.1
• Coordinates: 4490805..4491116 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZY40_RS21725
• Protein ID: WP_001259009.1
• Coordinates: 4491113..4491559 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZY40_RS21690 (4486463)	4486463..4487365	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
KZY40_RS21695 (4487362)	4487362..4487997	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
KZY40_RS21700 (4487994)	4487994..4488923	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
KZY40_RS21705 (4488970)	4488970..4489260	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
KZY40_RS21710 (4489261)	4489261..4489572	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
KZY40_RS21715 (4489790)	4489790..4490719	+	930	WP_001127705.1	alpha/beta hydrolase	-
KZY40_RS21720 (4490805)	4490805..4491116	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
KZY40_RS21725 (4491113)	4491113..4491559	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
KZY40_RS21730 (4491574)	4491574..4492515	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
KZY40_RS21735 (4492560)	4492560..4492997	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
KZY40_RS21740 (4492994)	4492994..4493866	-	873	WP_000921423.1	virulence factor BrkB family protein	-
KZY40_RS21745 (4493860)	4493860..4494459	-	600	WP_000965695.1	glucose-1-phosphatase	-
KZY40_RS21750 (4494650)	4494650..4495453	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
KZY40_RS21755 (4495487)	4495487..4496383	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T210744 WP_000558168.1 NZ_CP080091:4490805-4491116 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T210744 NZ_CP104604:227835-228119 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT210744 WP_001259009.1 NZ_CP080091:4491113-4491559 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT210744 NZ_CP104604:227597-227845 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A1R2S541