Detailed information of TA system TA210710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3993938..3994632
• Replicon: chromosome
• Accession: NZ_CP080085
• Organism: Escherichia coli strain 19SZHZ869Rt

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KZO18_RS19355
• Protein ID: WP_001263493.1
• Coordinates: 3994234..3994632 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KZO18_RS19350
• Protein ID: WP_000554757.1
• Coordinates: 3993938..3994231 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO18_RS19330 (3989578)	3989578..3990075	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
KZO18_RS19335 (3990291)	3990291..3992003	-	1713	Protein_3780	flagellar biosynthesis protein FlhA	-
KZO18_RS19340 (3991975)	3991975..3992760	+	786	WP_180760943.1	putative lateral flagellar export/assembly protein LafU	-
KZO18_RS19345 (3992831)	3992831..3993886	+	1056	WP_001226164.1	DNA polymerase IV	-
KZO18_RS19350 (3993938)	3993938..3994231	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KZO18_RS19355 (3994234)	3994234..3994632	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KZO18_RS19360 (3994642)	3994642..3995094	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KZO18_RS19365 (3995340)	3995340..3995543	+	204	Protein_3786	RtcB family protein	-
KZO18_RS19370 (3995542)	3995542..3996063	+	522	Protein_3787	peptide chain release factor H	-
KZO18_RS19375 (3996120)	3996120..3997577	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KZO18_RS19380 (3997838)	3997838..3998296	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (3998892)	3998892..3998972	+	81	NuclAT_12	-	-
- (3998892)	3998892..3998972	+	81	NuclAT_12	-	-
- (3998892)	3998892..3998972	+	81	NuclAT_12	-	-
- (3998892)	3998892..3998972	+	81	NuclAT_12	-	-
KZO18_RS19385 (3998388)	3998388..3999632	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T210710 WP_001263493.1 NZ_CP080085:3994234-3994632 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T210710 NZ_CP104598:c844273-843995 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATATTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT210710 WP_000554757.1 NZ_CP080085:3993938-3994231 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT210710 NZ_CP104598:c844536-844276 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6