Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3987125..3987666 Replicon chromosome
Accession NZ_CP080085
Organism Escherichia coli strain 19SZHZ869Rt

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag KZO18_RS19310 Protein ID WP_000615979.1
Coordinates 3987125..3987403 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KZO18_RS19315 Protein ID WP_000729704.1
Coordinates 3987406..3987666 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO18_RS19295 (3984707) 3984707..3985285 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KZO18_RS19300 (3985491) 3985491..3986258 + 768 WP_000333379.1 class II glutamine amidotransferase -
KZO18_RS19305 (3986229) 3986229..3986969 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KZO18_RS19310 (3987125) 3987125..3987403 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KZO18_RS19315 (3987406) 3987406..3987666 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KZO18_RS19320 (3987852) 3987852..3988625 + 774 WP_001353765.1 C40 family peptidase -
KZO18_RS19325 (3988767) 3988767..3989464 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
KZO18_RS19330 (3989578) 3989578..3990075 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
KZO18_RS19335 (3990291) 3990291..3992003 - 1713 Protein_3780 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T210709 WP_000615979.1 NZ_CP080085:c3987403-3987125 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T210709 NZ_CP104598:609637-609951 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT210709 WP_000729704.1 NZ_CP080085:c3987666-3987406 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT210709 NZ_CP104598:609401-609634 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References