Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2327218..2327945 Replicon chromosome
Accession NZ_CP080085
Organism Escherichia coli strain 19SZHZ869Rt

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZO18_RS11390 Protein ID WP_000550189.1
Coordinates 2327631..2327945 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZO18_RS11385 Protein ID WP_000560266.1
Coordinates 2327218..2327634 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO18_RS11375 (2322378) 2322378..2324729 + 2352 WP_000695486.1 alpha-glucosidase -
KZO18_RS11380 (2325155) 2325155..2327173 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZO18_RS11385 (2327218) 2327218..2327634 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZO18_RS11390 (2327631) 2327631..2327945 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZO18_RS11395 (2328229) 2328229..2329365 - 1137 WP_249768869.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZO18_RS11400 (2329450) 2329450..2329953 + 504 WP_001401153.1 M48 family metallopeptidase -
KZO18_RS11405 (2330030) 2330030..2330722 + 693 WP_001411307.1 vancomycin high temperature exclusion protein -
KZO18_RS11410 (2330801) 2330801..2331787 + 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T210706 WP_000550189.1 NZ_CP080085:c2327945-2327631 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T210706 NZ_CP104598:275102-275275 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT210706 WP_000560266.1 NZ_CP080085:c2327634-2327218 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT210706 NZ_CP104598:274778-275074 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References