Detailed information of TA system TA210663

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 143404..143658
• Replicon: plasmid p663Rt_IncR
• Accession: NZ_CP080076
• Organism: Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KZO16_RS23450
• Protein ID: WP_001312851.1
• Coordinates: 143509..143658 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	143404..143465 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO16_RS23435 (141358)	141358..142101	+	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-
KZO16_RS23440 (142156)	142156..142716	+	561	WP_000139310.1	fertility inhibition protein FinO	-
KZO16_RS23445 (142850)	142850..143062	+	213	WP_001541896.1	ANR family transcriptional regulator	-
- (143404)	143404..143465	-	62	NuclAT_1	-	Antitoxin
- (143404)	143404..143465	-	62	NuclAT_1	-	Antitoxin
- (143404)	143404..143465	-	62	NuclAT_1	-	Antitoxin
- (143404)	143404..143465	-	62	NuclAT_1	-	Antitoxin
KZO16_RS23450 (143509)	143509..143658	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KZO16_RS23455 (143926)	143926..144183	+	258	WP_001541895.1	replication regulatory protein RepA	-
KZO16_RS23460 (144286)	144286..144469	+	184	Protein_163	plasmid copy control protein CopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / blaTEM-1B / qnrS1 / tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12	-	1..144481	144481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T210663 WP_001312851.1 NZ_CP080076:143509-143658 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T210663 NZ_CP104586:c6191006-6190428 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
TCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGTTCGGCGCGACGCCTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT210663 NZ_CP080076:c143465-143404 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin