Detailed information of TA system TA210660

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104164..104433
• Replicon: plasmid p663Rt_IncR
• Accession: NZ_CP080076
• Organism: Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZO16_RS23235
• Protein ID: WP_001372321.1
• Coordinates: 104308..104433 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	104164..104229 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO16_RS23190	99238..99465	+	228	WP_071961421.1	hypothetical protein	-
KZO16_RS23195	99706..99912	+	207	WP_000275853.1	hypothetical protein	-
KZO16_RS23200	99938..100477	+	540	WP_032332913.1	single-stranded DNA-binding protein	-
KZO16_RS23205	100534..100767	+	234	WP_000005987.1	DUF905 family protein	-
KZO16_RS23210	100832..102790	+	1959	WP_223419851.1	ParB/RepB/Spo0J family partition protein	-
KZO16_RS23215	102845..103279	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KZO16_RS23220	103276..104038	+	763	Protein_115	plasmid SOS inhibition protein A	-
KZO16_RS23225	104007..104195	-	189	WP_001299721.1	hypothetical protein	-
-	104007..104231	+	225	NuclAT_0	-	-
-	104007..104231	+	225	NuclAT_0	-	-
-	104007..104231	+	225	NuclAT_0	-	-
-	104007..104231	+	225	NuclAT_0	-	-
-	104164..104229	-	66	-	-	Antitoxin
KZO16_RS23230	104217..104366	+	150	Protein_117	plasmid maintenance protein Mok	-
KZO16_RS23235	104308..104433	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZO16_RS23240	104653..104883	+	231	WP_001426396.1	hypothetical protein	-
KZO16_RS23245	104881..105054	-	174	Protein_120	hypothetical protein	-
KZO16_RS23250	105124..105384	+	261	WP_042046747.1	hypothetical protein	-
KZO16_RS23255	105478..106482	-	1005	WP_024257664.1	IS110 family transposase	-
KZO16_RS23260	106578..107003	+	426	WP_000422741.1	transposase	-
KZO16_RS23265	107000..107350	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
KZO16_RS23270	107381..108994	+	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / blaTEM-1B / qnrS1 / tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12	-	1..144481	144481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T210660 WP_001372321.1 NZ_CP080076:104308-104433 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T210660 NZ_CP104586:4827397-4827744 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT210660 NZ_CP080076:c104229-104164 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin