Detailed information of TA system TA210659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4522977..4523579
• Replicon: chromosome
• Accession: NZ_CP080075
• Organism: Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZO16_RS21670
• Protein ID: WP_000897305.1
• Coordinates: 4523268..4523579 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZO16_RS21665
• Protein ID: WP_000356397.1
• Coordinates: 4522977..4523267 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO16_RS21640 (4518922)	4518922..4519824	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KZO16_RS21645 (4519821)	4519821..4520456	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZO16_RS21650 (4520453)	4520453..4521382	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KZO16_RS21655 (4521712)	4521712..4521954	-	243	WP_001087409.1	protein YiiF	-
KZO16_RS21660 (4522173)	4522173..4522391	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KZO16_RS21665 (4522977)	4522977..4523267	-	291	WP_000356397.1	NadS family protein	Antitoxin
KZO16_RS21670 (4523268)	4523268..4523579	-	312	WP_000897305.1	hypothetical protein	Toxin
KZO16_RS21675 (4523808)	4523808..4524716	+	909	WP_001162704.1	alpha/beta hydrolase	-
KZO16_RS21680 (4524780)	4524780..4525721	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZO16_RS21685 (4525766)	4525766..4526203	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KZO16_RS21690 (4526200)	4526200..4527072	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KZO16_RS21695 (4527066)	4527066..4527665	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T210659 WP_000897305.1 NZ_CP080075:c4523579-4523268 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T210659 NZ_CP104586:4440193-4440603 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACTATCAAGAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGACACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT210659 WP_000356397.1 NZ_CP080075:c4523267-4522977 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT210659 NZ_CP104586:4439963-4440193 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGTTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7