Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 615172..615971 Replicon chromosome
Accession NZ_CP080075
Organism Escherichia coli strain 19SZHZ663Rt

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag KZO16_RS02980 Protein ID WP_000347266.1
Coordinates 615172..615636 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZO16_RS02985 Protein ID WP_001307405.1
Coordinates 615636..615971 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO16_RS02950 (610173) 610173..610607 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZO16_RS02955 (610625) 610625..611503 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
KZO16_RS02960 (611493) 611493..612272 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZO16_RS02965 (612283) 612283..612756 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZO16_RS02970 (612779) 612779..614059 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZO16_RS02975 (614308) 614308..615117 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZO16_RS02980 (615172) 615172..615636 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZO16_RS02985 (615636) 615636..615971 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZO16_RS02990 (616120) 616120..617690 - 1571 Protein_585 galactarate dehydratase -
KZO16_RS02995 (618065) 618065..619399 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZO16_RS03000 (619415) 619415..620185 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 615172..626626 11454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T210638 WP_000347266.1 NZ_CP080075:c615636-615172 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T210638 NZ_CP104582:c2497140-2496886 [Pseudomonas sp. GD03919]
GTGAAGCTGATCTGGTCTGAGCAAGCCTGGGACGATTATCTGTACTGGCAAGAGGCGGATAAGCGTATGGTCAAGAAGCT
CAACGAGCTGATCAAGGAGACCCGCCGCACCCCTTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGGCGGATCACCGCCGAGCACCGGTTTGTGTATTCGGTGACGGACGAGGCGCTGCTGATTGCCGCCTGC
CGTTATCACTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT210638 WP_001307405.1 NZ_CP080075:c615971-615636 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210638 NZ_CP104582:c2497388-2497137 [Pseudomonas sp. GD03919]
ATGCGCACTATCAGCTATACCGAAGCCCGGCAGAACCTGTCGGCCACCATGGTGCAGACCGTCGAGGATCGCGTGCCTAC
CCTCATTACCCGCCAGAACGGCGAGGCGTGCGTGCTGATGTCGCTGGAGGAGTACAACGCGCTGGAGGAAACGGCTTACC
TGCTGCGCTCGCCGGCCAACGCGCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCGTGGCCAGGGTGTCGAGCGGGAT
ATCGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References