Detailed information of TA system TA210633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3628079..3628773
• Replicon: chromosome
• Accession: NZ_CP080070
• Organism: Escherichia coli strain 19SZHZ713Rt

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KZO15_RS17400
• Protein ID: WP_001263493.1
• Coordinates: 3628079..3628477 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KZO15_RS17405
• Protein ID: WP_000554757.1
• Coordinates: 3628480..3628773 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3623739)	3623739..3623819	-	81	NuclAT_10	-	-
- (3623739)	3623739..3623819	-	81	NuclAT_10	-	-
- (3623739)	3623739..3623819	-	81	NuclAT_10	-	-
- (3623739)	3623739..3623819	-	81	NuclAT_10	-	-
KZO15_RS17370 (3623079)	3623079..3624323	-	1245	WP_000189541.1	esterase FrsA	-
KZO15_RS17375 (3624415)	3624415..3624873	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KZO15_RS17380 (3625134)	3625134..3626591	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
KZO15_RS17385 (3626648)	3626648..3627169	-	522	Protein_3421	peptide chain release factor H	-
KZO15_RS17390 (3627168)	3627168..3627371	-	204	Protein_3422	RtcB family protein	-
KZO15_RS17395 (3627617)	3627617..3628069	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KZO15_RS17400 (3628079)	3628079..3628477	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KZO15_RS17405 (3628480)	3628480..3628773	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KZO15_RS17410 (3628825)	3628825..3629880	-	1056	WP_001226164.1	DNA polymerase IV	-
KZO15_RS17415 (3629951)	3629951..3630736	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
KZO15_RS17420 (3630708)	3630708..3632420	+	1713	Protein_3428	flagellar biosynthesis protein FlhA	-
KZO15_RS17425 (3632745)	3632745..3633242	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / tssA / tssA / hcp1/tssD1	3627117..3659326	32209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T210633 WP_001263493.1 NZ_CP080070:c3628477-3628079 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T210633 NZ_CP104582:19237-19533 [Pseudomonas sp. GD03919]
GTGCCTAGCTTCAAGCTTGAGTTTCACGTCAAAGCCCGCAAGGAATGGGACAAGCTCGACGCGACCATTCGCCTCCAGTT
TGCCAAAAAGCTTAAAGAGCGCCTGAGCTCGCCTCGCATTGAGGCCGACAAGCTCAGTGGCATGGTTGACTGCTACAAGA
TCAAGCTTCGCTCTGCTGGCTACCGGCTTGTTTATCAGGTTGTCGATGACAAACTGGTTATAACGGTTGTGGCCGTTGGT
AAGCGTGAACGCTTTGAGGCCTACATTGCCGCTCAAAAACGAGTACAGCAGCAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT210633 WP_000554757.1 NZ_CP080070:c3628773-3628480 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT210633 NZ_CP104582:18966-19244 [Pseudomonas sp. GD03919]
ATGACCTTTCCAGTGCTGGCTGACGTCGCCGCTTCCGTTACTGACTTCAAGCGTGATCCGATGGGCACCATTCGCGAAGG
GCACGGTGAAACCGTCGTCATCCTCAACCGTAACGAACCGGCCTTTTACGCCGTGCCTCCAGCCCGCTATCAAGCCATGC
TCGAAATGCTCGATGACTTACGGCTGGCCGAAATTGTGCGTGCTCGCCAGGGGGGCGAAACAGTCTCGGTAGATATTGAC
GATCTGATCGCAGAAGCCGAGGCTGGCATCCGTGCCTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6