Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 701117..701844 Replicon chromosome
Accession NZ_CP080070
Organism Escherichia coli strain 19SZHZ713Rt

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KZO15_RS03405 Protein ID WP_000550189.1
Coordinates 701117..701431 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZO15_RS03410 Protein ID WP_097402472.1
Coordinates 701428..701844 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO15_RS03385 (697284) 697284..698270 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
KZO15_RS03390 (698349) 698349..699032 - 684 WP_001183046.1 vancomycin high temperature exclusion protein -
KZO15_RS03395 (699109) 699109..699612 - 504 WP_001295542.1 M48 family metallopeptidase -
KZO15_RS03400 (699697) 699697..700833 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KZO15_RS03405 (701117) 701117..701431 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KZO15_RS03410 (701428) 701428..701844 + 417 WP_097402472.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KZO15_RS03415 (701889) 701889..703907 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KZO15_RS03420 (704333) 704333..706684 - 2352 WP_000695489.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T210618 WP_000550189.1 NZ_CP080070:701117-701431 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T210618 NZ_CP104579:c3784831-3784406 [Pseudomonas oleovorans]
ATGTTCGTCCTCGACACTAATGTCGTGTCCGAACTGCGCAAGGCCAAGAGTGCCAAGGCCGACCCGCGCCTAGTGGCCTG
GGCTCAGCGCCTGCTGCCGGGGCAGTTGTTCATTTCCGCGATCACCGTGCTGGAGCTGGAAACCGGCGTGCTGCTAGTGG
AACGACGCGACCCGCAGCAGGGCCGCCTGCTGCGCTCCTGGCTCGATAGCCATGTACTACCCGGCTTCGCCGGACGCATC
CTGCCGGTCGACGCCGCCGTGGCGCAGCGTTGCGCGCAATTGCATGTCCCCGACCCACGCGCCGAACGCGATGCCTTGAT
CGTGGCCACGGCATTGGTGCATGGCATGACAGTCGCCACACGCAACATGGCGGACTTTCTGCCCACAGGTGTGGCGCTTA
TCAACCCGTGGGAGTTTGCTGGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15025.44 Da        Isoelectric Point: 4.4547

>AT210618 WP_097402472.1 NZ_CP080070:701428-701844 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHTKKLATRFGISPALFID

Download         Length: 417 bp

>AT210618 NZ_CP104579:c3785082-3784831 [Pseudomonas oleovorans]
ATGCAAACCATCCTTACCAGTCGCGAGTTCAATCAGGACGCCAGTGGTGCCAAGCGTGCTGCCAAACATGGGCCGGTCTT
TATCACGGATCGTGGCAAACCGGCTCATGTGCTGCTGAGCATCGAGGAATACCAGCGTCTGACCGGCGGCCCCGGCAGCA
TCATCGATCTGCTGGCCATGCCGACAGTCGAGGACATCGAGTTCGAGGCGCCGCGTGCGCAAGGTTTGTCGCGCCCGGCG
GATTTGAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References