Detailed information of TA system TA210615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4533785..4534387
• Replicon: chromosome
• Accession: NZ_CP080066
• Organism: Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZO14_RS21585
• Protein ID: WP_000897305.1
• Coordinates: 4534076..4534387 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZO14_RS21580
• Protein ID: WP_000356397.1
• Coordinates: 4533785..4534075 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO14_RS21555 (4529711)	4529711..4530613	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KZO14_RS21560 (4530610)	4530610..4531245	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZO14_RS21565 (4531242)	4531242..4532171	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KZO14_RS21570 (4532501)	4532501..4532743	-	243	WP_001086388.1	protein YiiF	-
KZO14_RS21575 (4532962)	4532962..4533180	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KZO14_RS21580 (4533785)	4533785..4534075	-	291	WP_000356397.1	NadS family protein	Antitoxin
KZO14_RS21585 (4534076)	4534076..4534387	-	312	WP_000897305.1	hypothetical protein	Toxin
KZO14_RS21590 (4534616)	4534616..4535524	+	909	WP_001162704.1	alpha/beta hydrolase	-
KZO14_RS21595 (4535588)	4535588..4536529	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZO14_RS21600 (4536574)	4536574..4537011	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KZO14_RS21605 (4537008)	4537008..4537880	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KZO14_RS21610 (4537874)	4537874..4538473	-	600	WP_063122015.1	glucose-1-phosphatase	-
KZO14_RS21615 (4538572)	4538572..4539357	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T210615 WP_000897305.1 NZ_CP080066:c4534387-4534076 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T210615 NZ_CP104579:2094141-2094434 [Pseudomonas oleovorans]
GTGCCACGATTGATTGTTACTGAAGGCGCGGCGAAGGGCTTGGAGCGTTGCCGGCGATTCCTCTCAGACAAAGATCCGCA
GGTCGCGCGCCGTGTTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCATTTC
CAGATCTGCCGGAGCTGCGTGAGCTCATCATCGAATTTGGTGACTCAGGTTACGTAGCGTTGTACCGCTATGAGCGTGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT210615 WP_000356397.1 NZ_CP080066:c4534075-4533785 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT210615 NZ_CP104579:2093881-2094153 [Pseudomonas oleovorans]
ATGGCCACTTCCATCAAAATTGATGACAAGCTAAAAGGCCGCATTCTGCAGTTGGCGAGCCAACGCCAGCGCTCGTCTCA
CTGGATCATGCGCGAGGCAATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAGCAGGAAGCCCTGGCAT
CGTGGGCGGCATATCAGGAAACCGGACGGCATTTGACCAGCCAAGAAACTCGGGCCTGGCTCGGTAGCTGGGGAACAGAT
ACTGAGGTAGAGCTTCCCAAGTGCCACGATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7