Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3715328..3715869 Replicon chromosome
Accession NZ_CP080066
Organism Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KZO14_RS17810 Protein ID WP_000615976.1
Coordinates 3715591..3715869 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KZO14_RS17805 Protein ID WP_000729704.1
Coordinates 3715328..3715588 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO14_RS17780 (3711111) 3711111..3711896 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
KZO14_RS17785 (3711868) 3711868..3713580 + 1713 Protein_3490 flagellar biosynthesis protein FlhA -
KZO14_RS17790 (3713685) 3713685..3713963 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KZO14_RS17795 (3713956) 3713956..3714312 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
KZO14_RS17800 (3714369) 3714369..3715142 - 774 WP_000543897.1 C40 family peptidase -
KZO14_RS17805 (3715328) 3715328..3715588 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KZO14_RS17810 (3715591) 3715591..3715869 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KZO14_RS17815 (3716025) 3716025..3716765 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KZO14_RS17820 (3716736) 3716736..3717503 - 768 WP_000333380.1 class II glutamine amidotransferase -
KZO14_RS17825 (3717709) 3717709..3718287 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T210612 WP_000615976.1 NZ_CP080066:3715591-3715869 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T210612 NZ_CP104579:956737-957012 [Pseudomonas oleovorans]
ATGTTGCCAATCATCTGGCGCGATAGCGCCAGAGCCGACTTGGCGGAAATCATCCGTTATATCGCTGATGAAGCCCCACA
GGCTGCCAGGCAGCTTAAGAATTACCTTGAATCTGCCGTCTTGCCGTTGGCTGAACACCCCTATCTGTATCGTTCCGGTC
GGGTTCCCGGCACTCGCGAGCTGGTCGCCCATCCCAACTATGTCTTGGTCTACAGGGTGGCGGCCGAGTGCATAGAAGTG
GTCAGCGTCCTTCATTCTCGCCAAGAATATCCTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT210612 WP_000729704.1 NZ_CP080066:3715328-3715588 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT210612 NZ_CP104579:956543-956737 [Pseudomonas oleovorans]
ATGAGTGTGCAACTTTCCCCCATCGTGTCGGAGTTCGAGACGCAGGAGCAGGCGGATAGTCATGATCGCTGGTTTCGCCG
CAAGGTAAAGGAGTCTTTGGATGACTCGCGTCCTGGTGTTCCGCATGACCAAGTGATGGCAGAAATGGAAGCCATCATTG
TTCAGGCTGAAGCTCGGCAGCGAGGCCGTGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References