Detailed information of TA system TA210598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1826289..1826791
• Replicon: chromosome
• Accession: NZ_CP080066
• Organism: Escherichia coli strain 19SZHZ803Rt

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: KZO14_RS08745
• Protein ID: WP_000767829.1
• Coordinates: 1826537..1826791 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KZO14_RS08740
• Protein ID: WP_001259255.1
• Coordinates: 1826289..1826540 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO14_RS08715 (1821467)	1821467..1822534	-	1068	WP_000080085.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KZO14_RS08720 (1822534)	1822534..1823604	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KZO14_RS08725 (1823601)	1823601..1824905	-	1305	WP_001593467.1	histidinol dehydrogenase	-
KZO14_RS08730 (1824911)	1824911..1825810	-	900	WP_094304651.1	ATP phosphoribosyltransferase	-
KZO14_RS08735 (1825956)	1825956..1826006	-	51	WP_001364200.1	his operon leader peptide	-
KZO14_RS08740 (1826289)	1826289..1826540	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KZO14_RS08745 (1826537)	1826537..1826791	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KZO14_RS08750 (1826874)	1826874..1827698	+	825	WP_000754763.1	SDR family oxidoreductase	-
KZO14_RS08755 (1827744)	1827744..1828673	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
KZO14_RS08760 (1828888)	1828888..1828950	+	63	WP_010723108.1	membrane protein YoeI	-
KZO14_RS08765 (1828940)	1828940..1830298	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KZO14_RS08770 (1830477)	1830477..1831535	+	1059	WP_023568741.1	thiosulfate utilization transporter TsuA/YeeE	-
KZO14_RS08775 (1831549)	1831549..1831776	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T210598 WP_000767829.1 NZ_CP080066:1826537-1826791 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T210598 NZ_CP104557:c5112878-5112579 [Pseudomonas promysalinigenes]
ATGAAAGAACAGTTTTATGATTACGACCCAGCCGAAGCGCTTACCGATGCAGAGTCCATCGCCATATTCATGGAGGATGC
GTTTGAGACGGGTGACGCGGCGTACATTGCGCAGGCCCTCGGTGTAGTGGCCCGCGCTAAGGGTATGACCACTATTGCCA
CTGAGGCGGGTGTTTCCCGGGAGCATCTGTATCGGTCGTTCTCGGCGAAAGGTAACCCTACGCTCAAGACGATGTTGGCT
GTGATGCGGGCGGTTGGTCTGGACCTGACCTCCCGCAGACACAGCAAACCGGCGCAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT210598 WP_001259255.1 NZ_CP080066:1826289-1826540 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT210598 NZ_CP104557:c5113177-5112875 [Pseudomonas promysalinigenes]
ATGAAGAGTATCAAGCAGACAGCCACCTACAGGAATTGGGAGCGCAAGCTACGGGACAAGCAGGCTAGGGCAATAATTGC
TGCACGCGTTTTTCGCCTGGCGCATGGTTTGCTTGGTGATGTCCAGCCAGTGGGTCAAGGTATCAGCGAACTGCGTATCC
ATCACGGCCCCGGTTACCGTATCTATTATCAGCAACGCGGTAATCAGTTGGTACTGTTGTTATGTGGTGGGGACAAGAGC
AGCCAGTCGCGAGATATCGAGACAGCGAAATCACTGGCCAGCCAGTGGAGTGATGATGAATGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0