Detailed information of TA system TA210587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4636460..4637062
• Replicon: chromosome
• Accession: NZ_CP080056
• Organism: Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZO13_RS22070
• Protein ID: WP_000897305.1
• Coordinates: 4636751..4637062 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZO13_RS22065
• Protein ID: WP_000356397.1
• Coordinates: 4636460..4636750 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZO13_RS22040 (4632405)	4632405..4633307	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KZO13_RS22045 (4633304)	4633304..4633939	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZO13_RS22050 (4633936)	4633936..4634865	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KZO13_RS22055 (4635195)	4635195..4635437	-	243	WP_001086388.1	protein YiiF	-
KZO13_RS22060 (4635656)	4635656..4635874	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KZO13_RS22065 (4636460)	4636460..4636750	-	291	WP_000356397.1	NadS family protein	Antitoxin
KZO13_RS22070 (4636751)	4636751..4637062	-	312	WP_000897305.1	hypothetical protein	Toxin
KZO13_RS22075 (4637291)	4637291..4638199	+	909	WP_001162704.1	alpha/beta hydrolase	-
KZO13_RS22080 (4638263)	4638263..4639204	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZO13_RS22085 (4639249)	4639249..4639686	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KZO13_RS22090 (4639683)	4639683..4640555	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KZO13_RS22095 (4640549)	4640549..4641148	-	600	WP_001295269.1	glucose-1-phosphatase	-
KZO13_RS22100 (4641247)	4641247..4642032	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T210587 WP_000897305.1 NZ_CP080056:c4637062-4636751 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T210587 NZ_CP104555:c660463-660251 [Vibrio sp. J502]
ATGCCGATTTCGGACATCATTGAAGTCGGCAACGTCATATCAAAGCTGCAAAGAGGCGAAAAACTCGATCCTAAAAATGT
TGATCATCCTTTAACTGGCGGATGGGTTGGATTCCGAGACTGTCACGTTAAACCAGATCTAGTGCTCATTTATCGCATTT
TTGATGGTCAACTCCAATTAGCTAGAATTGGCTCTCATAGTGATTTGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT210587 WP_000356397.1 NZ_CP080056:c4636750-4636460 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT210587 NZ_CP104555:c660791-660513 [Vibrio sp. J502]
ATGAGAACAGAAATGCTAAGTACTCGCATAGACCATGATACCAAAGTTGCTTTTACTAGCATCTGTGACGAAATGGGTCT
TAGTACATCACAAGCTATTAAAATTTTTGCAAAAGCCGTGATTAATCATGGTGGTATTCCATTTGATTTACGTGTACCTC
AACCAAATGAAACAACGATTTCTGCAATGAATGAACTCGTTCAAGGTCATGGACATAAAGCCGAATCTGTTGGCGCTATG
CTTACTGAGCTAACTGAAGGCAAAGTTAAGAATGTATAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7