Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 635801..636600 Replicon chromosome
Accession NZ_CP080056
Organism Escherichia coli strain 18SZHZ655Rt

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KZO13_RS03100 Protein ID WP_000347267.1
Coordinates 635801..636265 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZO13_RS03105 Protein ID WP_001307405.1
Coordinates 636265..636600 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZO13_RS03070 (630802) 630802..631236 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KZO13_RS03075 (631254) 631254..632132 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KZO13_RS03080 (632122) 632122..632901 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZO13_RS03085 (632912) 632912..633385 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZO13_RS03090 (633408) 633408..634688 - 1281 WP_000681936.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZO13_RS03095 (634937) 634937..635746 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KZO13_RS03100 (635801) 635801..636265 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZO13_RS03105 (636265) 636265..636600 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZO13_RS03110 (636749) 636749..638320 - 1572 WP_001273741.1 galactarate dehydratase -
KZO13_RS03115 (638695) 638695..640029 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZO13_RS03120 (640045) 640045..640815 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T210567 WP_000347267.1 NZ_CP080056:c636265-635801 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T210567 NZ_CP104541:69349-69501 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT210567 WP_001307405.1 NZ_CP080056:c636600-636265 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210567 NZ_CP104541:c69294-69237 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References