Detailed information of TA system TA210560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1027152..1027674
• Replicon: chromosome
• Accession: NZ_CP080013
• Organism: Macrococcus caseolyticus strain 19Msa1047

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KYI12_RS05195
• Protein ID: WP_219494767.1
• Coordinates: 1027152..1027424 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KYI12_RS05200
• Protein ID: WP_096076547.1
• Coordinates: 1027417..1027674 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYI12_RS05150 (KYI12_05145)	1022376..1022516	-	141	WP_186309442.1	hypothetical protein	-
KYI12_RS05155 (KYI12_05150)	1022609..1023073	+	465	WP_219494760.1	NUDIX domain-containing protein	-
KYI12_RS05160 (KYI12_05155)	1023159..1023410	+	252	WP_086042489.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KYI12_RS05165 (KYI12_05160)	1023410..1023670	+	261	WP_219494761.1	Txe/YoeB family addiction module toxin	-
KYI12_RS05170 (KYI12_05165)	1023671..1024120	-	450	WP_219494762.1	GNAT family N-acetyltransferase	-
KYI12_RS05175 (KYI12_05170)	1024203..1024943	+	741	WP_219494763.1	hypothetical protein	-
KYI12_RS05180 (KYI12_05175)	1024953..1025765	+	813	WP_219494764.1	phosphotransferase	-
KYI12_RS05185 (KYI12_05180)	1025875..1026360	+	486	WP_219494765.1	NUDIX domain-containing protein	-
KYI12_RS05190 (KYI12_05185)	1026366..1027124	+	759	WP_219494766.1	hypothetical protein	-
KYI12_RS05195 (KYI12_05190)	1027152..1027424	-	273	WP_219494767.1	Txe/YoeB family addiction module toxin	Toxin
KYI12_RS05200 (KYI12_05195)	1027417..1027674	-	258	WP_096076547.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KYI12_RS05205 (KYI12_05200)	1027805..1028977	-	1173	WP_219494768.1	MFS transporter	-
KYI12_RS05210 (KYI12_05205)	1029495..1029989	+	495	WP_219494769.1	isochorismatase family cysteine hydrolase	-
KYI12_RS05215 (KYI12_05210)	1030143..1030610	+	468	WP_219494770.1	hypothetical protein	-
KYI12_RS05220 (KYI12_05215)	1030806..1032068	+	1263	WP_219494771.1	Y-family DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10652.46 Da
• Isoelectric Point: 10.0258

>T210560 WP_219494767.1 NZ_CP080013:c1027424-1027152 [Macrococcus caseolyticus]
MPKKKILFYSSGFEDYTYWQTKDKTVSKKINKLIKSIMIDGANVGIGKPEKLLGNYTGCYSRRITHEHRLVYKIEDDSIC
ILQCRFHYKR

Nucleotide

• Length: 273 bp

>T210560 NZ_CP104540:c57090-56815 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCACCAGAACGGTGCAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9718.81 Da
• Isoelectric Point: 4.2805

>AT210560 WP_096076547.1 NZ_CP080013:c1027674-1027417 [Macrococcus caseolyticus]
MKVTTYSNARSNFRKIIDIVNEDCEPYTITTNTHNAVILSEHDYNSMIETLYLQSTPANAERLQKAIDESIDKNNLLQVE
IDLDA

Nucleotide

• Length: 258 bp

>AT210560 NZ_CP104540:c57374-57090 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCAATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGAAATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA