Detailed information of TA system TA210513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4522354..4522956
• Replicon: chromosome
• Accession: NZ_CP079992
• Organism: Escherichia coli strain KMS2001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: KYK85_RS21845
• Protein ID: WP_000897302.1
• Coordinates: 4522645..4522956 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KYK85_RS21840
• Protein ID: WP_000356397.1
• Coordinates: 4522354..4522644 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYK85_RS21810 (4518427)	4518427..4519329	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KYK85_RS21815 (4519326)	4519326..4519961	+	636	WP_025857205.1	formate dehydrogenase cytochrome b556 subunit	-
KYK85_RS21820 (4519958)	4519958..4520887	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
KYK85_RS21825 (4521103)	4521103..4521321	-	219	WP_001314326.1	CopG family transcriptional regulator	-
KYK85_RS21830 (4521717)	4521717..4521995	-	279	WP_001296612.1	hypothetical protein	-
KYK85_RS21835 (4522057)	4522057..4522269	-	213	WP_000197774.1	hypothetical protein	-
KYK85_RS21840 (4522354)	4522354..4522644	-	291	WP_000356397.1	NadS family protein	Antitoxin
KYK85_RS21845 (4522645)	4522645..4522956	-	312	WP_000897302.1	hypothetical protein	Toxin
KYK85_RS21850 (4523185)	4523185..4524093	+	909	WP_001331553.1	alpha/beta hydrolase	-
KYK85_RS21855 (4524157)	4524157..4525098	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KYK85_RS21860 (4525143)	4525143..4525580	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KYK85_RS21865 (4525577)	4525577..4526449	-	873	WP_025857204.1	virulence factor BrkB family protein	-
KYK85_RS21870 (4526443)	4526443..4527042	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T210513 WP_000897302.1 NZ_CP079992:c4522956-4522645 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T210513 NZ_CP104533:4249896-4250237 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCACTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT210513 WP_000356397.1 NZ_CP079992:c4522644-4522354 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT210513 NZ_CP104533:c4249901-4249825 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1P416