Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4237880..4238679 Replicon chromosome
Accession NZ_CP079992
Organism Escherichia coli strain KMS2001

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KYK85_RS20505 Protein ID WP_000347273.1
Coordinates 4238215..4238679 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KYK85_RS20500 Protein ID WP_001307405.1
Coordinates 4237880..4238215 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK85_RS20485 (4233665) 4233665..4234435 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
KYK85_RS20490 (4234451) 4234451..4235785 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KYK85_RS20495 (4236160) 4236160..4237731 + 1572 WP_001273753.1 galactarate dehydratase -
KYK85_RS20500 (4237880) 4237880..4238215 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KYK85_RS20505 (4238215) 4238215..4238679 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KYK85_RS20510 (4238734) 4238734..4239543 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KYK85_RS20515 (4239792) 4239792..4241072 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KYK85_RS20520 (4241095) 4241095..4241568 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KYK85_RS20525 (4241579) 4241579..4241950 + 372 Protein_4032 PTS sugar transporter subunit IIC -
KYK85_RS20530 (4241946) 4241946..4242503 + 558 Protein_4033 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4228732..4249663 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T210512 WP_000347273.1 NZ_CP079992:4238215-4238679 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T210512 NZ_CP104533:4125991-4126143 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT210512 WP_001307405.1 NZ_CP079992:4237880-4238215 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT210512 NZ_CP104533:c4125942-4125885 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References