210505

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2943510..2944012 Replicon chromosome
Accession NZ_CP079992
Organism Escherichia coli strain KMS2001

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag KYK85_RS14430 Protein ID WP_000767829.1
Coordinates 2943510..2943764 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KYK85_RS14435 Protein ID WP_001259255.1
Coordinates 2943761..2944012 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK85_RS14400 (2938525) 2938525..2938752 - 228 WP_000234896.1 sulfurtransferase TusA -
KYK85_RS14405 (2938766) 2938766..2939824 - 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
KYK85_RS14410 (2940003) 2940003..2941361 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KYK85_RS14415 (2941351) 2941351..2941413 - 63 WP_010723108.1 membrane protein YoeI -
KYK85_RS14420 (2941628) 2941628..2942557 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
KYK85_RS14425 (2942603) 2942603..2943427 - 825 WP_000754737.1 SDR family oxidoreductase -
KYK85_RS14430 (2943510) 2943510..2943764 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KYK85_RS14435 (2943761) 2943761..2944012 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KYK85_RS14440 (2944295) 2944295..2944345 + 51 WP_001364200.1 his operon leader peptide -
KYK85_RS14445 (2944491) 2944491..2945390 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
KYK85_RS14450 (2945396) 2945396..2946700 + 1305 WP_000009594.1 histidinol dehydrogenase -
KYK85_RS14455 (2946697) 2946697..2947767 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
KYK85_RS14460 (2947767) 2947767..2948834 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T210505 WP_000767829.1 NZ_CP079992:c2943764-2943510 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T210505 NZ_CP104533:2220118-2220220 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT210505 WP_001259255.1 NZ_CP079992:c2944012-2943761 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT210505 NZ_CP104533:c2220258-2219990 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References