Detailed information of TA system TA210481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4522353..4522955
• Replicon: chromosome
• Accession: NZ_CP079991
• Organism: Escherichia coli strain KMS2005

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: KYK86_RS21865
• Protein ID: WP_000897302.1
• Coordinates: 4522644..4522955 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KYK86_RS21860
• Protein ID: WP_000356397.1
• Coordinates: 4522353..4522643 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYK86_RS21830 (4518426)	4518426..4519328	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KYK86_RS21835 (4519325)	4519325..4519960	+	636	WP_025857205.1	formate dehydrogenase cytochrome b556 subunit	-
KYK86_RS21840 (4519957)	4519957..4520886	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
KYK86_RS21845 (4521102)	4521102..4521320	-	219	WP_001314326.1	CopG family transcriptional regulator	-
KYK86_RS21850 (4521716)	4521716..4521994	-	279	WP_001296612.1	hypothetical protein	-
KYK86_RS21855 (4522056)	4522056..4522268	-	213	WP_000197774.1	hypothetical protein	-
KYK86_RS21860 (4522353)	4522353..4522643	-	291	WP_000356397.1	NadS family protein	Antitoxin
KYK86_RS21865 (4522644)	4522644..4522955	-	312	WP_000897302.1	hypothetical protein	Toxin
KYK86_RS21870 (4523184)	4523184..4524092	+	909	WP_001331553.1	alpha/beta hydrolase	-
KYK86_RS21875 (4524156)	4524156..4525097	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KYK86_RS21880 (4525142)	4525142..4525579	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KYK86_RS21885 (4525576)	4525576..4526448	-	873	WP_025857204.1	virulence factor BrkB family protein	-
KYK86_RS21890 (4526442)	4526442..4527041	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T210481 WP_000897302.1 NZ_CP079991:c4522955-4522644 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T210481 NZ_CP104526:1008653-1008988 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT210481 WP_000356397.1 NZ_CP079991:c4522643-4522353 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT210481 NZ_CP104526:1008405-1008653 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: S1P416