Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1144018..1144712 Replicon chromosome
Accession NZ_CP079991
Organism Escherichia coli strain KMS2005

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KYK86_RS05485 Protein ID WP_001263489.1
Coordinates 1144314..1144712 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KYK86_RS05480 Protein ID WP_000554758.1
Coordinates 1144018..1144311 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK86_RS05460 (1139650) 1139650..1140147 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KYK86_RS05465 (1140371) 1140371..1142083 - 1713 Protein_1074 flagellar biosynthesis protein FlhA -
KYK86_RS05470 (1142055) 1142055..1142840 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KYK86_RS05475 (1142911) 1142911..1143966 + 1056 WP_001226164.1 DNA polymerase IV -
KYK86_RS05480 (1144018) 1144018..1144311 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KYK86_RS05485 (1144314) 1144314..1144712 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KYK86_RS05490 (1144722) 1144722..1145174 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
KYK86_RS05495 (1145492) 1145492..1145698 + 207 Protein_1080 RtcB family protein -
KYK86_RS05500 (1145694) 1145694..1146215 + 522 Protein_1081 peptide chain release factor H -
KYK86_RS05505 (1146272) 1146272..1147729 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KYK86_RS05510 (1147990) 1147990..1148448 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -
- (1149044) 1149044..1149124 + 81 NuclAT_4 - -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T210458 WP_001263489.1 NZ_CP079991:1144314-1144712 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T210458 NZ_CP104523:2222679-2222855 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT210458 WP_000554758.1 NZ_CP079991:1144018-1144311 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT210458 NZ_CP104523:2222899-2223315 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A090J8B1


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References