Detailed information of TA system TA210446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe(toxin)
• Location: 1253266..1253777
• Replicon: chromosome
• Accession: NZ_CP079984
• Organism: Macrococcus bohemicus strain 19Msa0383

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KYI13_RS06530
• Protein ID: WP_219497413.1
• Coordinates: 1253517..1253777 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KYI13_RS06525
• Protein ID: WP_086042489.1
• Coordinates: 1253266..1253517 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYI13_RS06495 (KYI13_06500)	1248310..1249314	-	1005	WP_188017441.1	cation diffusion facilitator family transporter	-
KYI13_RS06500 (KYI13_06505)	1249533..1249925	+	393	WP_133353150.1	sporulation protein	-
KYI13_RS06505 (KYI13_06510)	1250167..1251111	+	945	WP_219497410.1	LacI family DNA-binding transcriptional regulator	-
KYI13_RS06510 (KYI13_06515)	1251195..1251653	+	459	WP_219497411.1	NUDIX domain-containing protein	-
KYI13_RS06515 (KYI13_06520)	1251830..1252567	+	738	WP_165958555.1	class I SAM-dependent methyltransferase	-
KYI13_RS06520 (KYI13_06525)	1252983..1253180	+	198	WP_219497412.1	hypothetical protein	-
KYI13_RS06525 (KYI13_06530)	1253266..1253517	+	252	WP_086042489.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KYI13_RS06530 (KYI13_06535)	1253517..1253777	+	261	WP_219497413.1	Txe/YoeB family addiction module toxin	Toxin
KYI13_RS06535 (KYI13_06540)	1253778..1254227	-	450	WP_219497414.1	GNAT family N-acetyltransferase	-
KYI13_RS06540 (KYI13_06545)	1254310..1255050	+	741	WP_219497415.1	hypothetical protein	-
KYI13_RS06545 (KYI13_06550)	1255060..1255950	+	891	WP_219497416.1	phosphotransferase	-
KYI13_RS06550 (KYI13_06555)	1256135..1256620	+	486	WP_219497417.1	NUDIX domain-containing protein	-
KYI13_RS06555 (KYI13_06560)	1256626..1257387	+	762	WP_219497418.1	hypothetical protein	-
KYI13_RS06560 (KYI13_06565)	1257473..1258645	-	1173	WP_219497419.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-76)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-59)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10273.94 Da
• Isoelectric Point: 10.2531

>T210446 WP_219497413.1 NZ_CP079984:1253517-1253777 [Macrococcus bohemicus]
MYKIVMKSSVKKDLKKIKNTHLETNFLKIIKQLKENPYNNNHSFEKLIPPIKGFYSRRINVQHRVVYTVNDEDKIATIYS
AYGHYE

Nucleotide

• Length: 261 bp

>T210446 NZ_CP104521:4932486-4932797 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9590.65 Da
• Isoelectric Point: 4.0373

>AT210446 WP_086042489.1 NZ_CP079984:1253266-1253517 [Macrococcus bohemicus]
MNTYTPTSARKDFFNIIKSVNEDSKEIYIAPTKIGEKGAVLVGEDDWNAIQETLFLLEKGIAYQIEQRKDEEEIDFDSTW
DNI

Nucleotide

• Length: 252 bp

>AT210446 NZ_CP104521:4932798-4933088 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG