Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3603032..3603573 Replicon chromosome
Accession NZ_CP079924
Organism Escherichia sp. TC-EC600-tetX4

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KXD89_RS17535 Protein ID WP_000615983.1
Coordinates 3603295..3603573 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KXD89_RS17530 Protein ID WP_000729703.1
Coordinates 3603032..3603292 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXD89_RS17510 (3598707) 3598707..3599492 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KXD89_RS17515 (3599464) 3599464..3601176 + 1713 Protein_3430 flagellar biosynthesis protein FlhA -
KXD89_RS17520 (3601400) 3601400..3601897 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KXD89_RS17525 (3602073) 3602073..3602822 - 750 WP_000056849.1 C40 family peptidase -
KXD89_RS17530 (3603032) 3603032..3603292 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KXD89_RS17535 (3603295) 3603295..3603573 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KXD89_RS17540 (3603729) 3603729..3604469 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KXD89_RS17545 (3604440) 3604440..3605207 - 768 WP_000333380.1 class II glutamine amidotransferase -
KXD89_RS17550 (3605413) 3605413..3605991 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T210395 WP_000615983.1 NZ_CP079924:3603295-3603573 [Escherichia sp. TC-EC600-tetX4]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T210395 NZ_CP104512:39545-39697 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT210395 WP_000729703.1 NZ_CP079924:3603032-3603292 [Escherichia sp. TC-EC600-tetX4]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT210395 NZ_CP104512:c39490-39433 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References