210394

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3596835..3597529 Replicon chromosome
Accession NZ_CP079924
Organism Escherichia sp. TC-EC600-tetX4

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag KXD89_RS17495 Protein ID WP_001263489.1
Coordinates 3596835..3597233 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KXD89_RS17500 Protein ID WP_000554758.1
Coordinates 3597236..3597529 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXD89_RS17470 (3592516) 3592516..3593496 + 981 WP_000019403.1 IS5-like element IS5 family transposase -
KXD89_RS17475 (3593818) 3593818..3595275 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
KXD89_RS17480 (3595332) 3595332..3595853 - 522 Protein_3423 peptide chain release factor H -
KXD89_RS17485 (3595849) 3595849..3596055 - 207 Protein_3424 RtcB family protein -
KXD89_RS17490 (3596373) 3596373..3596825 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
KXD89_RS17495 (3596835) 3596835..3597233 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KXD89_RS17500 (3597236) 3597236..3597529 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KXD89_RS17505 (3597581) 3597581..3598636 - 1056 WP_001226164.1 DNA polymerase IV -
KXD89_RS17510 (3598707) 3598707..3599492 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
KXD89_RS17515 (3599464) 3599464..3601176 + 1713 Protein_3430 flagellar biosynthesis protein FlhA -
KXD89_RS17520 (3601400) 3601400..3601897 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T210394 WP_001263489.1 NZ_CP079924:c3597233-3596835 [Escherichia sp. TC-EC600-tetX4]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T210394 NZ_CP104512:2265-2540 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT210394 WP_000554758.1 NZ_CP079924:c3597529-3597236 [Escherichia sp. TC-EC600-tetX4]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT210394 NZ_CP104512:1981-2265 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References