Detailed information of TA system TA210357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1296799..1297385
• Replicon: chromosome
• Accession: NZ_CP079903
• Organism: Pseudomonas juntendi strain K37-3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A6I2K6L8
• Locus tag: KZA81_RS05935
• Protein ID: WP_029885077.1
• Coordinates: 1297107..1297385 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KZA81_RS05930
• Protein ID: WP_029885078.1
• Coordinates: 1296799..1297098 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZA81_RS05905 (KZA81_05860)	1292685..1293494	+	810	WP_009686964.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-
KZA81_RS05910 (KZA81_05865)	1293566..1293976	-	411	WP_260778558.1	SufE family protein	-
KZA81_RS05915 (KZA81_05870)	1293973..1295178	-	1206	WP_029885081.1	cysteine desulfurase	-
KZA81_RS05920 (KZA81_05875)	1295260..1296294	-	1035	WP_260778559.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
KZA81_RS05925 (KZA81_05880)	1296326..1296673	-	348	WP_054881204.1	ArsC family reductase	-
KZA81_RS05930 (KZA81_05885)	1296799..1297098	-	300	WP_029885078.1	type II toxin-antitoxin system antitoxin GraA	Antitoxin
KZA81_RS05935 (KZA81_05890)	1297107..1297385	-	279	WP_029885077.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZA81_RS05940 (KZA81_05895)	1297634..1299280	+	1647	WP_029885076.1	Na+/H+ antiporter	-
KZA81_RS05945 (KZA81_05900)	1299825..1301021	-	1197	WP_029888073.1	succinyldiaminopimelate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.72 Da
• Isoelectric Point: 7.1344

>T210357 WP_029885077.1 NZ_CP079903:c1297385-1297107 [Pseudomonas juntendi]
MIQSFSCADTEALFVTGKTRRWSDIKSVAERKLAMLDAATELRDLRSPPGNRLEALSGNRAGQHSIRVNDQWRLCFTWTD
NGPANVEIIDYH

Nucleotide

• Length: 279 bp

>T210357 NZ_CP104503:3640154-3640327 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10903.58 Da
• Isoelectric Point: 6.4603

>AT210357 WP_029885078.1 NZ_CP079903:c1297098-1296799 [Pseudomonas juntendi]
MLKNGMRPIHPGEVLREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQRHGEEIIGAVQRLVA

Nucleotide

• Length: 300 bp

>AT210357 NZ_CP104503:3639830-3640126 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A6I2K6L8