Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4383958..4384637 Replicon chromosome
Accession NZ_CP079891
Organism Escherichia fergusonii strain 5zf15-2-1

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KYD87_RS21665 Protein ID WP_223666017.1
Coordinates 4383958..4384260 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag KYD87_RS21670 Protein ID WP_000806442.1
Coordinates 4384296..4384637 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYD87_RS21640 (4379334) 4379334..4380986 + 1653 WP_182212215.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
KYD87_RS21645 (4381024) 4381024..4381527 - 504 WP_000667001.1 hypothetical protein -
KYD87_RS21650 (4381524) 4381524..4382180 - 657 WP_223666016.1 hypothetical protein -
KYD87_RS21655 (4382348) 4382348..4382827 - 480 WP_182212213.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
KYD87_RS21660 (4383031) 4383031..4383825 - 795 WP_181588489.1 TraB/GumN family protein -
KYD87_RS21665 (4383958) 4383958..4384260 + 303 WP_223666017.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KYD87_RS21670 (4384296) 4384296..4384637 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
KYD87_RS21675 (4384695) 4384695..4387199 - 2505 WP_182212212.1 copper-exporting P-type ATPase CopA -
KYD87_RS21680 (4387506) 4387506..4388795 + 1290 WP_148048125.1 APC family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11825.40 Da        Isoelectric Point: 10.2638

>T210351 WP_223666017.1 NZ_CP079891:4383958-4384260 [Escherichia fergusonii]
MTQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T210351 NZ_CP104503:c1933012-1932677 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT210351 WP_000806442.1 NZ_CP079891:4384296-4384637 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT210351 NZ_CP104503:c1933260-1933012 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References