Detailed information of TA system TA210329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1995765..1996459
• Replicon: chromosome
• Accession: NZ_CP079891
• Organism: Escherichia fergusonii strain 5zf15-2-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: B7LNG9
• Locus tag: KYD87_RS10220
• Protein ID: WP_001263502.1
• Coordinates: 1995765..1996163 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KYD87_RS10225
• Protein ID: WP_000554758.1
• Coordinates: 1996166..1996459 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYD87_RS10190 (1990894)	1990894..1992351	+	1458	WP_182212348.1	cytosol nonspecific dipeptidase	-
KYD87_RS10195 (1992360)	1992360..1992641	+	282	WP_182212347.1	hypothetical protein	-
KYD87_RS10200 (1992650)	1992650..1993174	-	525	WP_141095971.1	metal-dependent hydrolase	-
KYD87_RS10205 (1993236)	1993236..1993850	-	615	WP_182212346.1	peptide chain release factor H	-
KYD87_RS10210 (1993847)	1993847..1994986	-	1140	WP_182212345.1	RNA ligase RtcB family protein	-
KYD87_RS10215 (1995303)	1995303..1995755	-	453	WP_001059848.1	GNAT family N-acetyltransferase	-
KYD87_RS10220 (1995765)	1995765..1996163	-	399	WP_001263502.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KYD87_RS10225 (1996166)	1996166..1996459	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KYD87_RS10230 (1996511)	1996511..1997566	-	1056	WP_001226156.1	DNA polymerase IV	-
KYD87_RS10235 (1997637)	1997637..1998422	-	786	WP_182212343.1	putative lateral flagellar export/assembly protein LafU	-
KYD87_RS10240 (1998394)	1998394..2000106	+	1713	Protein_2008	flagellar biosynthesis protein FlhA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1976705..2008440	31735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15486.90 Da
• Isoelectric Point: 8.9401

>T210329 WP_001263502.1 NZ_CP079891:c1996163-1995765 [Escherichia fergusonii]
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T210329 NZ_CP104500:2336704-2336880 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT210329 WP_000554758.1 NZ_CP079891:c1996459-1996166 [Escherichia fergusonii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT210329 NZ_CP104500:2336926-2337342 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: B7LNG9

Antitoxin

• AlphaFold DB: S1QAE3