Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 518900..519462 Replicon chromosome
Accession NZ_CP079891
Organism Escherichia fergusonii strain 5zf15-2-1

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag KYD87_RS02660 Protein ID WP_000605673.1
Coordinates 519184..519462 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KYD87_RS02655 Protein ID WP_000781370.1
Coordinates 518900..519184 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYD87_RS02640 (514259) 514259..517306 + 3048 WP_223666161.1 formate dehydrogenase-N subunit alpha -
KYD87_RS02645 (517319) 517319..518203 + 885 WP_148048370.1 formate dehydrogenase N subunit beta -
KYD87_RS02650 (518196) 518196..518849 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
KYD87_RS02655 (518900) 518900..519184 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KYD87_RS02660 (519184) 519184..519462 - 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KYD87_RS02665 (519648) 519648..520658 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
KYD87_RS02670 (520793) 520793..522490 - 1698 WP_000433464.1 malate dehydrogenase -
KYD87_RS02675 (522647) 522647..522784 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KYD87_RS02680 (522886) 522886..523101 - 216 WP_000495766.1 biofilm-dependent modulation protein -
KYD87_RS02685 (523444) 523444..523875 + 432 WP_000152299.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T210324 WP_000605673.1 NZ_CP079891:c519462-519184 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T210324 NZ_CP104500:1937650-1937901 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT210324 WP_000781370.1 NZ_CP079891:c519184-518900 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT210324 NZ_CP104500:1937898-1938152 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References