Detailed information of TA system TA210296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3622375..3623069
• Replicon: chromosome
• Accession: NZ_CP079884
• Organism: Escherichia fergusonii strain 6S41-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: B7LNG9
• Locus tag: KYC43_RS17830
• Protein ID: WP_001263502.1
• Coordinates: 3622375..3622773 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KYC43_RS17835
• Protein ID: WP_000554758.1
• Coordinates: 3622776..3623069 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYC43_RS17800 (3617504)	3617504..3618961	+	1458	WP_223671718.1	cytosol nonspecific dipeptidase	-
KYC43_RS17805 (3619009)	3619009..3619251	+	243	WP_223671720.1	hypothetical protein	-
KYC43_RS17810 (3619260)	3619260..3619784	-	525	WP_141095971.1	metal-dependent hydrolase	-
KYC43_RS17815 (3619846)	3619846..3620460	-	615	WP_182212346.1	peptide chain release factor H	-
KYC43_RS17820 (3620457)	3620457..3621596	-	1140	WP_182212345.1	RNA ligase RtcB family protein	-
KYC43_RS17825 (3621913)	3621913..3622365	-	453	WP_001059848.1	GNAT family N-acetyltransferase	-
KYC43_RS17830 (3622375)	3622375..3622773	-	399	WP_001263502.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KYC43_RS17835 (3622776)	3622776..3623069	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KYC43_RS17840 (3623121)	3623121..3624176	-	1056	WP_001226156.1	DNA polymerase IV	-
KYC43_RS17845 (3624247)	3624247..3625032	-	786	WP_182212343.1	putative lateral flagellar export/assembly protein LafU	-
KYC43_RS17850 (3625004)	3625004..3626716	+	1713	Protein_3485	flagellar biosynthesis protein FlhA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15486.90 Da
• Isoelectric Point: 8.9401

>T210296 WP_001263502.1 NZ_CP079884:c3622773-3622375 [Escherichia fergusonii]
MRVFKTKLIRLQLTAKELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T210296 NZ_CP104489:c43060-42779 [Salmonella enterica]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT210296 WP_000554758.1 NZ_CP079884:c3623069-3622776 [Escherichia fergusonii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT210296 NZ_CP104489:c43301-43050 [Salmonella enterica]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: B7LNG9

Antitoxin

• AlphaFold DB: S1QAE3