Detailed information of TA system TA021022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Gp49-HTH_37
• Location: 5271863..5272271
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PLU_RS25785
• Protein ID: WP_082303157.1
• Coordinates: 5272143..5272271 (-)
• Length: 43 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PLU_RS22280
• Protein ID: WP_011148593.1
• Coordinates: 5271863..5272150 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS22260 (plu4509)	5267962..5269017	+	1056	WP_011148589.1	hypothetical protein	-
PLU_RS22265 (plu4510)	5269014..5270600	+	1587	WP_011148590.1	AMP-binding protein	-
PLU_RS22270 (plu4511)	5270597..5271220	+	624	WP_011148591.1	CDP-alcohol phosphatidyltransferase family protein	-
PLU_RS22275 (plu4512)	5271210..5271752	+	543	WP_011148592.1	CDP-archaeol synthase	-
PLU_RS22280 (plu4513)	5271863..5272150	-	288	WP_011148593.1	helix-turn-helix domain-containing protein	Antitoxin
PLU_RS25785	5272143..5272271	-	129	WP_082303157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PLU_RS22285 (plu4514)	5272435..5273580	-	1146	WP_011148594.1	ABC transporter permease	-
PLU_RS22290 (plu4515)	5273580..5274725	-	1146	WP_011148595.1	ABC transporter permease	-
PLU_RS22295 (plu4516)	5274733..5275704	-	972	WP_011148596.1	efflux RND transporter periplasmic adaptor subunit	-
PLU_RS22300 (plu4517)	5275942..5276556	+	615	Protein_4457	ECF transporter S component	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-37)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 43 a.a.
• Molecular weight: 4960.83 Da
• Isoelectric Point: 8.9777

>T21022 WP_082303157.1 NC_005126:c5272271-5272143 [Photorhabdus laumondii subsp. laumondii TTO1]
IHYLFSQGKQVYILRCFVKKTDKTPPSEIELALKRLEDMTYG

Nucleotide

• Length: 129 bp

>T21022 NC_005126:c5272271-5272143 [Photorhabdus laumondii subsp. laumondii TTO1]
ATTCATTATCTTTTTTCACAAGGGAAGCAGGTTTACATTTTACGATGCTTTGTGAAGAAAACAGATAAAACACCACCATC
TGAAATAGAGTTAGCACTGAAACGATTGGAGGATATGACATATGGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10724.44 Da
• Isoelectric Point: 9.8357

>AT21022 WP_011148593.1 NC_005126:c5272150-5271863 [Photorhabdus laumondii subsp. laumondii TTO1]
MAKVTPISHSEVRKRLLNTPEAIQAYVEATEEYELLEQLTEWREKAGLKKIDIAKRMGINPSAVTRIEKNVTKASWHTLK
RYAAACGVELSLTAK

Nucleotide

• Length: 288 bp

>AT21022 NC_005126:c5272150-5271863 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGGCTAAAGTAACTCCTATCTCACATTCAGAAGTGAGAAAGCGACTTCTTAATACACCGGAAGCTATACAAGCCTATGT
TGAGGCTACAGAAGAATATGAGCTGCTGGAACAACTCACTGAGTGGCGGGAAAAGGCGGGTCTAAAGAAAATAGATATAG
CTAAACGGATGGGAATTAATCCGTCTGCGGTTACCCGTATTGAGAAGAATGTAACGAAAGCTAGTTGGCACACACTTAAA
CGTTATGCTGCTGCATGTGGTGTTGAGTTATCTTTAACTGCGAAGTAA