Detailed information of TA system TA021018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4669337..4670004
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7N0B2
• Locus tag: PLU_RS19740
• Protein ID: WP_011148113.1
• Coordinates: 4669337..4669660 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PLU_RS19745
• Protein ID: WP_011148114.1
• Coordinates: 4669708..4670004 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS19725 (plu3979)	4665714..4667558	+	1845	WP_011148111.1	RNA polymerase sigma factor RpoD	-
PLU_RS26195 (plu3980)	4668381..4668953	-	573	WP_011148112.1	hypothetical protein	-
PLU_RS19740 (plu3981)	4669337..4669660	+	324	WP_011148113.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PLU_RS19745 (plu3982)	4669708..4670004	+	297	WP_011148114.1	helix-turn-helix domain-containing protein	Antitoxin
PLU_RS26935	4670430..4670630	+	201	WP_125026254.1	hypothetical protein	-
PLU_RS19750 (plu3985)	4671018..4671953	-	936	Protein_3937	hypothetical protein	-
PLU_RS19755 (plu3986)	4672068..4673012	-	945	WP_011148118.1	class I SAM-dependent methyltransferase	-
PLU_RS19760 (plu3987)	4673150..4673683	-	534	WP_011148119.1	isopentenyl-diphosphate Delta-isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (34-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-92)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12550.65 Da
• Isoelectric Point: 10.1702

>T21018 WP_011148113.1 NC_005126:4669337-4669660 [Photorhabdus laumondii subsp. laumondii TTO1]
MEYLVFIETPVFSRERAELLTDDEFRLLQEHLLKNHEQGSTISATGGCKKIRWQRERIGKRGGVRIIYYAITKTGRLYLL
LIYPKNKKDDLTPKEKEVLKAISNKLQ

Nucleotide

• Length: 324 bp

>T21018 NC_005126:4669337-4669660 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGGAATATCTGGTTTTTATTGAAACTCCCGTATTTAGTAGAGAACGAGCAGAACTGTTAACCGATGATGAATTTAGGCT
ATTACAGGAGCACTTACTAAAGAATCACGAGCAGGGTAGTACCATCAGCGCTACTGGAGGATGTAAAAAGATTCGTTGGC
AGCGAGAAAGGATAGGAAAACGTGGCGGAGTAAGAATTATCTACTATGCCATAACCAAAACTGGGAGGCTCTATCTCCTT
TTGATTTACCCTAAAAATAAAAAAGATGACTTAACTCCAAAAGAAAAAGAGGTACTTAAAGCCATCAGTAACAAATTACA
GTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11269.92 Da
• Isoelectric Point: 6.2274

>AT21018 WP_011148114.1 NC_005126:4669708-4670004 [Photorhabdus laumondii subsp. laumondii TTO1]
MDKRLFNDLVESMNQMVAIEKGELSVFDENIHYHRLPDVKNLRETFGLKQSEFAEAVGVSTSLIQSWEQHRRIPSGSSLK
LLKMLERNPALINELSTL

Nucleotide

• Length: 297 bp

>AT21018 NC_005126:4669708-4670004 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGGACAAAAGACTTTTTAACGATTTGGTTGAAAGCATGAATCAGATGGTAGCAATTGAAAAAGGCGAACTTTCTGTTTT
TGATGAAAATATCCATTACCATCGCCTGCCTGATGTAAAAAACTTACGTGAAACCTTTGGCTTAAAACAAAGTGAATTTG
CCGAAGCTGTTGGCGTTTCTACATCTCTAATTCAAAGCTGGGAACAGCACCGCCGCATTCCTTCTGGTTCATCACTGAAA
CTACTTAAGATGCTTGAACGTAATCCTGCGCTTATTAATGAATTAAGCACACTTTAA

Structures

Toxin

• AlphaFold DB: A0A6L9JJS0