Detailed information of TA system TA210159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3192992..3193644
• Replicon: chromosome
• Accession: NZ_CP079823
• Organism: Aeromonas veronii strain HD6454

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KW556_RS14850
• Protein ID: WP_103828992.1
• Coordinates: 3192992..3193342 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KW556_RS14855
• Protein ID: WP_042046566.1
• Coordinates: 3193342..3193644 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW556_RS14825 (KW556_14775)	3189473..3190531	+	1059	WP_259583689.1	integrase arm-type DNA-binding domain-containing protein	-
KW556_RS14830 (KW556_14780)	3190498..3190926	-	429	WP_259583690.1	hypothetical protein	-
KW556_RS14835 (KW556_14785)	3190927..3191364	-	438	WP_259583691.1	DUF4065 domain-containing protein	-
KW556_RS14840 (KW556_14790)	3191328..3192002	-	675	WP_259583692.1	hypothetical protein	-
KW556_RS14845 (KW556_14795)	3191994..3192944	+	951	WP_103828991.1	DUF932 domain-containing protein	-
KW556_RS14850 (KW556_14800)	3192992..3193342	+	351	WP_103828992.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW556_RS14855 (KW556_14805)	3193342..3193644	+	303	WP_042046566.1	XRE family transcriptional regulator	Antitoxin
KW556_RS14860 (KW556_14810)	3193821..3194822	+	1002	WP_103828993.1	YqaJ viral recombinase family protein	-
KW556_RS14865 (KW556_14815)	3194819..3195757	+	939	WP_029303610.1	hypothetical protein	-
KW556_RS14870 (KW556_14820)	3195759..3196106	-	348	WP_029303609.1	helix-turn-helix transcriptional regulator	-
KW556_RS14875 (KW556_14825)	3196556..3196780	+	225	WP_259583693.1	hypothetical protein	-
KW556_RS14880 (KW556_14830)	3196877..3197509	+	633	WP_233768813.1	hypothetical protein	-
KW556_RS14885 (KW556_14835)	3197594..3198154	+	561	WP_029303605.1	lecithin retinol acyltransferase family protein	-
KW556_RS14890 (KW556_14840)	3198192..3198617	+	426	WP_259583694.1	DUF2787 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3189473..3196106	6633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13075.07 Da
• Isoelectric Point: 8.0312

>T210159 WP_103828992.1 NZ_CP079823:3192992-3193342 [Aeromonas veronii]
MWTVKTTELFNAWFDVQDEATQEKVLAGLLALAQGGPSVGRPLVDTIKGSRFTNLKELRVQHQGEPLRAFFAFDPLRQAI
VLCAGKKGGNEKRFYKEMLPLAEAQYARHLAELEEK

Nucleotide

• Length: 351 bp

>T210159 NZ_CP104442:c2764905-2764654 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11179.90 Da
• Isoelectric Point: 5.2187

>AT210159 WP_042046566.1 NZ_CP079823:3193342-3193644 [Aeromonas veronii]
MGRTLDDLLASRSEESRQRIQEMATELLLEVRIQALREELEVSQVELAKALGVSQPAVVAMEQRGSDIKLSTMKRYVEAM
GGKLRVDVELPTGRHIGFNV

Nucleotide

• Length: 303 bp

>AT210159 NZ_CP104442:c2764657-2764403 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA