Detailed information of TA system TA210152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13599..14256
• Replicon: plasmid pS465MC_p1
• Accession: NZ_CP079808
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WRC02_S465MC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: KWL80_RS27040
• Protein ID: WP_000270043.1
• Coordinates: 13906..14256 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KWL80_RS27035
• Protein ID: WP_000124640.1
• Coordinates: 13599..13901 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KWL80_RS26985 (KWL80_26960)	9233..9661	+	429	WP_000591076.1	hypothetical protein	-
KWL80_RS26990 (KWL80_26965)	9719..10078	+	360	WP_000422769.1	hypothetical protein	-
KWL80_RS26995 (KWL80_26970)	10078..10524	+	447	WP_000919343.1	hypothetical protein	-
KWL80_RS27000 (KWL80_26975)	10521..11039	+	519	WP_000210757.1	nitrite reductase	-
KWL80_RS27005 (KWL80_26980)	11039..11269	+	231	WP_000972665.1	hypothetical protein	-
KWL80_RS27010 (KWL80_26985)	11256..12113	+	858	WP_001167036.1	hypothetical protein	-
KWL80_RS28540	12139..12330	+	192	WP_001270409.1	hypothetical protein	-
KWL80_RS27020 (KWL80_26995)	12333..12860	+	528	WP_004201083.1	thermonuclease family protein	-
KWL80_RS27025 (KWL80_27000)	12918..13190	+	273	WP_001043046.1	HU family DNA-binding protein	-
KWL80_RS27030 (KWL80_27005)	13279..13572	+	294	WP_001239998.1	hypothetical protein	-
KWL80_RS27035 (KWL80_27010)	13599..13901	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
KWL80_RS27040 (KWL80_27015)	13906..14256	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KWL80_RS27045 (KWL80_27020)	14419..14967	+	549	WP_001061573.1	hypothetical protein	-
KWL80_RS27050 (KWL80_27025)	15308..15502	+	195	WP_000343597.1	hypothetical protein	-
KWL80_RS27055 (KWL80_27030)	15513..15884	+	372	WP_000516918.1	hypothetical protein	-
KWL80_RS27060 (KWL80_27035)	15877..16347	+	471	WP_001281821.1	hypothetical protein	-
KWL80_RS27065 (KWL80_27040)	16362..16697	-	336	WP_000683477.1	hypothetical protein	-
KWL80_RS27070 (KWL80_27045)	16794..17282	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
KWL80_RS27075 (KWL80_27050)	17285..17782	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-62 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E)	-	1..161754	161754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T210152 WP_000270043.1 NZ_CP079808:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T210152 NZ_CP104442:c1945033-1944926 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT210152 WP_000124640.1 NZ_CP079808:c13901-13599 [Klebsiella pneumoniae subsp. pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT210152 NZ_CP104442:1945086-1945147 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9